摘要
为了探究川射干提取物对急性肺损伤的防治效果及其对TLR4/NF-κB信号通路相关蛋白表达的影响,试验将24只雌性Balb/c小鼠随机分为空白对照(CTR)组、模型(LPS)组、地塞米松(DEX)组及川射干提取物(ITR)组,每组6只。ITR组每只每天灌胃12.5 mg/mL川射干提取物0.2 mL,其他组灌胃等体积生理盐水,连续7 d。末次给药1 h后,LPS组、DEX组和ITR组每只小鼠腹腔注射1 mg/mL脂多糖0.2 mL造模,CTR组腹腔注射等体积生理盐水。DEX组于造模前1小时每只小鼠腹腔注射0.5 mg/mL地塞米松0.2 mL。造模6 h后采集血液,制备血清;处死各组小鼠,分别采集肺泡灌洗液(BALF)和肺脏,通过ELISA法检测血清和肺泡灌洗液中趋化因子1(CXCL1)、肿瘤坏死因子-α(TNF-α)、白细胞介素(IL)-1β、IL-6、IL-8和IL-10的含量;对肺脏组织进行H.E.染色,观察肺脏病理变化;通过Western-blot检测Toll样受体4(TLR4)、核因子κB(NF-κB)信号通路相关蛋白的表达情况。结果表明:与LPS组相比,ITR组血清和肺泡灌洗液中CXCL1、TNF-α、IL-1β、IL-6、IL-8含量均显著或极显著下降(P<0.05或P<0.01),IL-10含量显著或极显著升高(P<0.05或P<0.01);与LPS组比较,ITR组肺泡内炎症细胞浸润程度明显减轻,仅局部肺泡腔内出现少量巨噬细胞或淋巴细胞浸润。与LPS组比较,ITR组肺脏TLR4、MyD88、TRIF和NF-κB p-p65蛋白表达量显著或极显著降低(P<0.05或P<0.01)。说明川射干提取物可通过调节TLR4/NF-κB信号通路缓解脂多糖诱导的小鼠急性肺损伤。
In order to investigate the prevention and treatment effect of Iris tectorum rhizoma extract on acute lung injury and its effect on the expression of the proteins associated with TLR4/NF-κB signaling pathway,in the experiment,twenty-four female Balb/c mice were randomly divided into blank control(CTR)group,model(LPS)group,dexamethasone(DEX)group and Iris tectorum rhizoma extract(ITR)group,with 6 mice in each group.In the ITR group,0.2 mL Iris tectorum rhizoma extract of 12.5 mg/mL per day was given by gavage,and the other groups were given by gavage with an equal volume of normal saline for 7 days.1 h after the last dose,each mouse in the LPS,DEX and ITR groups was injected with 0.2 mL lipopolysaccharide of 1 mg/mL intraperitoneally,and the CTR group was intraperitoneally injected with an equal volume of normal saline.In the DEX group,0.2 mL dexamethasone of 0.5 mg/mL was injected intraperitoneally per mouse 1 h before modeling.After 6 h of modeling,each group of mice was sacrificed;serum,alveolar lavage fluid(BALF),and lung were collected;the contents of chemokine 1(CXCL1),tumor necrosis factor-α(TNF-α),interleukin(IL)-1β,IL-6,IL-8 and IL-10 in serum and BALF were detected by ELISA.H.E.staining was performed on lung tissue,and pathological changes of lung were observed.The expression of the proteins related to the Toll-like receptor 4(TLR4)and nuclear factorκB(NF-κB)signaling pathway was detected by Western-blot.The results showed that compared with the LPS group,the contents of CXCL1,TNF-α,IL-1β,IL-6 and IL-8 in serum and alveolar lavage fluid in the ITR group decreased significantly or extremely significantly(P<0.05 or P<0.01),and the content of IL-10 increased significantly or extremely significantly(P<0.05 or P<0.01).Compared with the LPS group,the degree of inflammatory cell infiltration in the alveoli in the ITR group was significantly reduced,and only a small number of macrophage or lymphocyte infiltration appeared in the local alveolar cavity.Compared with the LPS group,the expression of TLR4,MyD88,TRIF and NF-κB p-p65 proteins in the ITR group was significantly or extremely significantly reduced(P<0.05 or P<0.01).The results suggested that Iris tectorum rhizoma extract could alleviate LPS-induced acute lung inflammation in mice by regulating the TLR4/NF-κB signaling pathway.
作者
张敏
李思聪
王斌
李旭廷
梁歌
ZHANG Min;LI Sicong;WANG Bin;LI Xuting;LIANG Ge(Sichuan Animal Sciences Academy,Chengdu 610066,China;Animal Breeding and Genetics Key laboratory of Sichuan Provincial,Chengdu 610066,China)
出处
《黑龙江畜牧兽医》
北大核心
2023年第23期103-109,137,共8页
Heilongjiang Animal Science And veterinary Medicine
基金
国家现代农业产业技术体系四川省兽药创新团队项目(SCCXTD-2020-18)
四川省基本科研业务项目(SASA202104)
畜禽新型中兽药产业化关键技术转化应用项目(2022JDZH0028)
畜禽重大疫病防控技术研究与示范项目(SASA2023CZYX006)。