基于高通量测序的甜高粱青贮饲料中微生物群落分析

Microbial diversity analysis of sweet sorghum silage using High-Throughput sequencing

【目的】研究甜高粱青贮饲料的微生物多样性,为青贮饲料的生产和贮藏提供理论基础。【方法】以甜高粱青贮饲料为研究对象,利用高通量测序技术分析甜高粱青贮饲料中微生物群落结构和多样性,研究添加剂对甜高粱青贮饲料中微生物群落的影响。【结果】甜高粱青贮饲料中细菌优势菌门为厚壁菌门、变形菌门、拟杆菌门、蓝细菌门、酸杆菌门,优势菌属主要为乳酸杆菌属、肠杆菌属、乳球菌属、棒状杆菌;真菌群落优势菌门为子囊菌门。【结论】不同处理的甜高粱青贮饲料之间微生物菌群组成相近,但相对丰度存在明显差异,青贮菌剂降低了甜高粱青贮饲料中微生物的相对丰度。

【Objective】To clarify the microbial diversity of sweet sorghum silage in the hope of providing a theoretical basis for silage production and storage.【Methods】In this study,the microbial diversity of silage from two varieties with or without the addition of silage additives were analyzed using high-throughput sequencing.【Results】The dominant bacterial phyla in sweet sorghum silage were Firmicutes,Proteobacteria,Bacteroidetes,Cyanobacteria and Acidobacteria,and the dominant bacterial genera were Lactobacillus,Enterobacter,Lactococcus and Corynebacterium.Ascomycota was the dominant phylum in fungal community.【Conclusion】The microbial flora composition of sweet sorghum silage is similar among different treatments,but the relative abundance is significantly different.Silage microbial agent reduces the relative abundance of microbial flora in sweet sorghum silage.