Lsd1对小鼠调节性T细胞发育及活化的影响

Effects of Lsd1 on the development and activation of regulatory T cells in mice

目的探讨赖氨酸特异性去甲基化酶1(lysine-specific demethylase 1,Lsd1)对调节性T细胞(regulatory T cells,Treg)发育及活化的影响。方法利用条件性敲除胸腺T细胞中Lsd1的C57BL/6小鼠(Lsd1 fl/fl Lck-Cre),取对照组及敲除组小鼠胸腺、脾和淋巴结,通过流式细胞术检测Treg的数量和比例,并统计效应Treg(activated effector Treg cell,eTreg)和静息Treg(naive-like central Treg cell,cTreg)比例(eTreg/cTreg)的变化。结果与对照组相比,敲除组小鼠胸腺指数减小,胸腺中CD4+T细胞减少,但Treg的数量和比例增加。在外周,脾和淋巴结中Treg的比例增加,eTreg/cTreg比值增加。结论条件性敲除T细胞中Lsd1促进胸腺中Treg的发育,并可能引起脾和淋巴结中Treg的活化。

Objective To investigate the effects of lysine-specific demethylase 1(Lsd1)in the development and activation of regulatory T cells(Treg).Methods Thymi,spleens,and lymph nodes were isolated from control and T cell-specific Lsd1 conditional knockout(Lsd1 fl/fl Lck-Cre)mice.The numbers and proportions of Tregs and the ratio changes of eTreg/cTreg were measured by flow cytometry.Results Compared with the control mice,the thymus indices and numbers of thymic CD4+T cells decreased in the Lsd1 knockout mice,but the numbers and proportions of thymic Treg increased.In the spleen and lymph nodes,the proportions of Treg increased,so did the eTreg/cTreg ratio.Conclusion Conditional knockout of Lsd1 in T cells promotes the development of Tregs in the thymus and the activation of Tregs in the spleen and lymph nodes.