新型同种异体骨修复骨缺损对动物生理生化指标的影响

Effects of new bone allograft on physiological and biochemical indices of animals

目的探究新型同种异体骨修复骨缺损时对受试动物生理(体质量、血常规)及生物化学指标的影响。方法用雄性新西兰兔构建骨缺损动物模型,将60只动物随机分为实验组、对照组和空白对照组。在双侧股骨制备深度约为1 mm的骨缺损。实验组(供试品)和对照组(对照品)动物左股骨缺损区填充皮质骨粒,右股骨缺损区填充松质骨小块。空白组动物不填充任何材料。各组动物分别于术后1周、4周、8周和12周采血,行血常规和血生化检查。结果术后1周,3组动物体质量均有轻度下降,1周之后,实验动物体质量多数呈增长趋势。在各实验周期内,各组之间体质量变化比较差异无统计学意义(P>0.05)。血常规检测结果显示,植入供试品对动物的血液系统、造血功能并无不良影响。生化检查结果提示供试品的植入可能对受试动物的生物化学反应及代谢产生一定影响,考虑为术后机体的适应性反应,并未造成病理影响。结论该新型同种异体骨对受试动物体质量、血常规及生化指标无不良影响,是一种安全的植入修复材料。

Objective To study the effects of new bone allogeneic repair on physiological and biochemical indexes of the tested animals.Methods Male New Zealand rabbits were used to construct the animal model of bone injury,and 60 rabbits were randomly divided into experimental group,control group and blank control group.At the outer edge of proximal femur,the bone defect with a depth of about 1 mm was prepared by precision grinding mechanism with a diameter of 4 mm ball head.In the experimental group and control group,the left femoral defect was filled with cortical bone particles,and the right femoral defect was filled with cancellous bone fragments.Animals in the blank group underwent the same operation as described above without any material filling.Blood samples were collected at 1,4,8 and 12 weeks after operation,and blood routine and blood biochemical examination were performed.Results One week after surgery,the weight of animals in both groups decreased slightly,and one week later,most of the experimental animals showed an increasing trend.However,there was no significant difference in body weight change among all groups in each experimental period(P>0.05).The results of blood routine test showed that the implant had no adverse effect on the blood system and hematopoietic function of the animals.The results of biochemical examination suggest that the implantation of the test product may have some effects on the biochemical reaction and metabolism of the recipient animal,but this is still a adaptive reaction of the body and does not cause pathological effects.Conclusion This type of allograft bone has no adverse effects on the body weight,blood routine and biochemical indexes of the tested animals,and is a safe implant repair material.