转基因抗虫耐除草剂玉米自交系LG11的获得及抗性分析

Acquisition and resistance analysis of transgenic Maize Inbred Line LG11 with insect and herbicide resistance

虫害是影响我国玉米产量和品质的重要限制因子,转Bt (Bacillus thuringiensis)基因玉米具有很好的抗虫性,能有效减少化学杀虫剂的使用,深受种植者欢迎。但是大面积持续种植转基因玉米,会引发靶标害虫产生抗性,“多基因”策略是阻止或延缓靶标害虫抗性种群发生的主要治理策略之一。本课题组利用人工合成方法将Cry1Ab和Vip3Aa蛋白的主要结构域组合形成融合蛋白M2cryAb-vip3Aa,这2种杀虫蛋白在进化上没有同源性且杀虫机理存在差别,能有效降低靶标害虫产生抗性的几率。本研究拟通过转基因方法将m2cryAb-vip3Aa和bar基因串联导入受体材料,并以骨干自交系昌7-2为回交亲本进行回交转育,获得抗虫耐除草剂且拥有优良农艺性状的转基因玉米新材料,并对抗虫性和耐除草剂抗性进行分析。研究结果将丰富现有的抗虫耐除草剂玉米种质资源,并为玉米田间害虫防治和杂草治理提供新的解决方案。

Insect pests are an important limiting factor affecting the yield and quality of maize.Bacillus thuringiensis transgenic maize has good insect resistance and can reduce the use of chemical insecticides effectively,which is very popular among growers.However,a large area of continuous planting of transgenic corn will lead to the resistance of target pests.“Multi-gene”strategy is one of the management strategies to prevent or delay the occurrence of resistant populations of target pests.The anti-insect vector constructed in this study is a fusion protein M2cryAb-vip3Aa formed by combining the main structural domains of Cry1Ab and Vip3Aa proteins using synthetic methods.These two insecticidal proteins had no evolutionary homology and different insecticidal mechanisms,which could effectively reduce the resistance probability of target pests.In this study,to obtain a new transgenic maize material with insect resistance and herbicide resistance and excellent agronomic traits,and analyze its insect resistance and herbicide resistance,m2cryAb-vip3Aa and bar genes were introduced into the recipient materials in series by transgenic method,and Chang 7-2,a backbone inbred line,was used as the backcross parent for backcross transfer.The results of this study enriches the existing germplasm resources of insect-resistant and herbicide-tolerant maize,and provides new solutions for pest control and weed control in maize fields.