ATP快速荧光检测技术在医院PIVAS感染控制领域的应用和展望

Enhanced Application and Prospects of Rapid Fluorescence Detection of ATP in PIVAS Infection Control within Hospital Settings

目的提高静脉用药调配中心(PIVAS)感染控制的水平,加强环境微生物快速检测及管理能力,降低静脉用药受微生物污染的风险,保障成品输液质量与临床用药安全。方法通过对手持式ATP生物荧光检测仪的技术原理、发展历程、操作细节及不同应用领域的分析,探讨未来该技术在PIVAS的应用场景。结果相比平皿计数法等传统方法,手持式ATP生物荧光检测仪在医院PIVAS应用具有可现场采样和检测,可立即得出结果,可测定非可培养菌(VBNC),可追踪污染源等优势。但也存在准确度、灵敏度不足,试剂控温要求较苛刻,人为操作误差大,价格较高等局限性。结论ATP生物荧光检测技术填补了传统细菌培养方法检测周期较长的空白。该方法虽存在一定局限性,但随着技术的发展和普及,将越来越多的应用于包括PIVAS在内的医院感染控制领域。

OBJECTIVE To enhance the level of infection control in Pharmacy Intravenous Admixture Services(PIVAS),strengthen the capability for rapid environmental microbial testing and management,reduce the risk of intravenous drug contamination by microorganisms,and ensure the quality of finished infusion products and the safety of clinical medication.METHODS By analyzing the technical principles,developmental history,operational details,and diverse application areas of handheld ATP bioluminescence assay devices,this study explores the potential application scenarios of this technology in PIVAS in the future.RESULTS Compared to conventional methods such as standard plate count methods,handheld ATP bioluminescence assay devices offer several advantages for their application in PIVAS within hospitals.These advantages include on-site sampling and testing,immediate results,the ability to detect viable but non-culturable(VBNC)bacteria,and the capability to trace the source of contamination.However,there are limitations as well,such as insufficient accuracy and sensitivity,stringent reagent temperature control requirements,significant potential for human operational errors,and higher costs.CONCLUSION The ATP bioluminescence assay fills the gap of long detection periods in traditional bacterial culture methods.While this method has certain limitations,with the advancement and widespread adoption of technology,it will be increasingly applied in hospital infection control areas,including PIVAS.