含油废水中产脂肪酶微生物的筛选和酶学性质研究

Screening of Lipase-producing Microorganisms from Oily Wastewater and Study of Their Enzymatic Properties

采用罗丹明B平板初筛和摇瓶发酵复筛法从含油废水中筛选出产脂肪酶活性较高的菌株,并对活性最高的菌株F-11进行酶活性条件分析,发现F-11属革兰氏阴性杆菌。对F-11菌株所产脂肪酶进行酶催化特性分析发现,该脂肪酶属中温碱性脂肪酶,反应转速为150 r/min时,其最佳初始pH为7.5,最佳培养温度为30℃,菌株酶活性最高为10.96 U/mL,稳定性良好。

Lipase-producing microorganisms from oily wastewater were screened for high lipase activity by primary sieving in Rhodamine B plate and secondary sieving in shaking flask fermentation,and the highest active strain F-11 was analyzed for enzyme activity conditions,and it was found that F-11 was a gram-negative bacillus.The enzyme catalytic characteristics of the lipase produced by strain F-11 showed that the lipase was a medium-temperature alkaline lipase,with an optimal initial pH of 7.5 and an optimal incubation temperature of 30℃at a reaction speed of 150 r/min,and the highest enzyme activity of the strain was 10.96 U/mL,with a good stability.