慢痞消组方中药对嗜黏蛋白阿克曼菌、大肠杆菌和粪肠球菌的增殖影响及药效规律初探

A preliminary study on the effffects and pharmacological principles of traditional Chinese medicines in the Manpixiao formula on the proliferation of akkermansia muciniphila,escherichia coli,and enterococcus faecalis

目的探究慢痞消12味中药对胃癌前病变(PLGC)大鼠特征菌嗜黏蛋白阿克曼菌(Akk)、大肠杆菌(E.coli)和粪肠球菌(E.fa)增殖的调控作用及其药效规律。方法分别经镜检、基因组测序分析,对三株菌进行形态、基因特征鉴定;采用比浊法检测不同浓度慢痞消12味中药提取物对三株菌增殖的影响;采用聚类分析各味中药对三株菌调节作用的药效规律。结果经镜检及基因组学测序,鉴定三株菌质量合格。白术、丹参、甘草、玫瑰花、砂仁、太子参和玉竹能明显促进Akk增殖;白术、丹参、玫瑰花、乌梅、香橼、玉竹和紫苏梗能明显抑制E.coli增殖;甘草、玫瑰花、砂仁、太子参、乌梅、香橼、玉竹和紫苏梗能明显抑制E.fa增殖。12味中药对三株菌的调节作用聚类分析结果分为三类,具有理气活血、祛瘀解毒的祛邪作用的是:紫苏梗、香橼、延胡索、白花蛇舌草、玫瑰花、丹参、砂仁;具有益气和中、养阴生津的扶正作用的是:玉竹、太子参、甘草、乌梅;具有补气燥湿和中,即扶正祛邪的作用的是白术。结论慢痞消组方中药对PLGC特征菌具有一定的调控作用,即促进益生菌Akk增殖、抑制致病菌E.coli及E.fa增殖,与中药药效特点存在相关性。

Objective To investigate the regulatory effects and pharmacological regularities of traditional Chinese medicines in the Manpixiao formula on the proliferation of akkermansia muciniphila(Akk),escherichia coli(E.coli),and enterococcus faecalis(E.fa),three characteristic strains of precancerous lesions of gastric cancer(PLGC)rats.Methods The morphology and genetic characteristics of the three bacterial strains were identified by microscopy and genome sequencing analysis.The turbidimetric method was used to detect the effects of different concentrations of the water extracts of each of the 12 traditional Chinese medicines(TCMs)in Manpixiao decoction on the proliferation of the three bacterial strains.A clustering analysis was performed to determine the pharmacological regularity of each TCM in regulating the proliferation of the three bacterial strains.Results The quality of the three bacterial strains were identified as qualified through microscopy and genomic sequencing analysis.Largehead atractylodes rhizome,Radix salviae miltiorrhizae,liquorice root,rose flower,villous amomum fruit,heterophylly falsestarwort root,and fragrant solomonseal rhizome could promote the proliferation of Akk,while largehead atractylodes rhizome,Radix salviae miltiorrhizae,rose flower,smoked plum,citron fruit,fragrant solomonseal rhizome,and perilla stem could inhibit the proliferation of E.coli,and liquorice root,rose flower,villous amomum fruit,heterophylly falsestarwort root,smoked plum,citron fruit,fragrant solomonseal rhizome,and perilla stem could inhibit the proliferation of E.fa.The pharmacological regularities of the 12 TCMs in Manpixiao decoction on the regulation of the three bacterial strains were clustered into three categories:the qi-regulating,blood-activating,stasis-removing,and toxin-removing effects of dispelling the pathogenic factors:perilla stem,citron fruit,yanhusuo,hedyotis,rose flower,Radix salviae miltiorrhizae,and villous amomum fruit;the tonifying yang,nourishing yin,and generating fluid to invigorate health effects:fragrant solomonseal rhizome,heterophylly falsestarwort root,liquorice root,and smoked plum;the effects of tonifying qi,drying dampness,and assisting in the correction of the regulation to eliminate pathogenic factors:largehead atractylodes rhizome.Conclusion The Manpixiao formula has a certain regulatory effect on the characteristic bacteria of PLGC,namely,promoting the proliferation of probiotics Akk and inhibiting the proliferation of pathogenic bacteria E.coli and E.fa.