PRPF18对肝癌的影响及药敏分析

Effect and drug sensitivity analysis of PRPF18 on HCC

目的:分析PRPF18对肝癌细胞株MHCC97H增殖、迁移、侵袭和凋亡的影响及药敏分析。方法:通过生物信息学来分析PRPF18在肝细胞癌中的表达、预后及药物敏感性。qRT-PCR验证PRPF18在肝癌细胞与正常肝细胞中的表达情况。通过瞬时转染在MHCC97H细胞中敲低PRPF18,qRT-PCR验证敲低效果。CCK-8实验和克隆形成实验分析敲低PRPF18对MHCC97H细胞增殖的影响。Transwell实验和划痕实验分析敲低PRPF18对MHCC97H细胞迁移和侵袭的影响。Hoechst 33342染色和线粒体膜电位测定分析敲低PRPF18对MHCC97H细胞凋亡的影响。结果:PRPF18在HCC中高表达,且与患者预后显著相关。PRPF18高表达的患者对替吡法尼和舒尼替尼有更高的敏感性。敲低PRPF18后MHCC97H细胞的增殖、迁移和侵袭能力相比对照组明显降低。敲低PRPF18降低线粒体膜电位,促进细胞凋亡。结论:PRPF18在肝癌中有较好的预后价值。敲低PRPF18能抑制MHCC97H细胞的增殖、迁移和侵袭,促进细胞凋亡。

Objective:To analyze the effects of PRPF18 on the proliferation,migration,invasion and apoptosis of hepato⁃cellular carcinoma cell line MHCC97H and drug sensitivity analysis.Methods:The expression,prognosis and drug sensitivity of PRPF18 in hepatocellular carcinoma have been analyzed by using bioinformatics.The expression of PRPF18 in HCC and normal hepatocytes was verified by qRT⁃PCR.PRPF18 was silenced by transient transfection in MHCC97H cells,and the silencing ef⁃fect was verified by qRT⁃PCR.CCK⁃8 assay and colony formation assay were used to analyze the effect of silencing PRPF18 on the proliferation of MHCC97H cells.The effects of silencing PRPF18 on the migration and invasion of MHCC97H cells were ana⁃lyzed by Transwell assay and scratch assay.Hoechst 33342 staining and mitochondrial membrane potential assay were used to ana⁃lyze the effect of silencing PRPF18 on apoptosis of MHCC97H cells.Results:PRPF18 was highly expressed in HCC and signifi⁃cantly associated with the prognosis of patients.Patients with high expression of PRPF18 had higher sensitivity to tipifarnib and sunitinib. After silencing PRPF18, the proliferation, migration and invasion of MHCC97H cells were significantly lower thanthose of the control group. Silencing PRPF18 reduced mitochondrial membrane potential and promoted apoptosis. Conclusion:PRPF18 has a good prognostic value in HCC. Silencing PRPF18 may inhibit the proliferation, migration and invasion ofMHCC97H cells and promote apoptosis.