粘附剂与附着基质对马尾藻受精卵附着的影响

Effects of Adhesives and Attachment Substrates on the Attach of Sargassum Zygotes

初选6种粘附剂与马尾藻受精卵混合后,培养观察其附着萌发情况,筛选使受精卵成活率最高的粘附剂;使用4%海藻酸钠、3%CaCl_(2)混合受精卵涂覆在3种粗糙度不同的附着基质上,观察受精卵附着密度,筛选对受精卵附着最有利的粘附剂与基质组合。结果表明:(1)涂抹海藻酸钠的受精卵附着密度达259 ind.·cm^(-2),成活率9.4%;超支化聚合物胶粘剂(HBPA)包裹的受精卵附着密度虽低,但材料黏度大、粘附性强、溶解慢,是潜在的室外移植粘附材料;(2)砖块、水泥板、PVC基质上喷涂3%CaCl_(2)的附着密度达86、48、8 ind.·cm^(-2),成活率分别为52%、29%、5%;实验说明基面越粗糙,受精卵附着数量越多。因此,在大型海藻修复时可投放基面粗糙度大、有颗粒凹点的藻礁与人工粘附剂相结合的方法移植大型海藻。

Six kinds of adhesives were mixed with zygotes of Sargassum and cultured to observe the attachment germination,screening of the highest survival of the zygote adhesion agents.Zygotes mixed with 4%alginate and 3%CaCl_(2) were coated on three attachment substratum with different roughness,the adhesion density of zygotes was observed to screen the most favorable combination of adhesive and attachment substrate for adhesion of zygotes.The results show that:(1)The attachment density of zygotes coated with alginate reached 259 ind.·cm^(-2),and the survival rate was 9.4%.The attachment density of hyperbranched polymer adhesive(HBPA)coated zygotes was low,however,the material has large viscosity,strong adhesion and slow dissolution,HBPA is a potential adhesion material for outdoor transplantation.(2)The attachment density of 3%CaCl_(2) sprayed on brick,cement board and PVC substrate is 86,48 and 8 ind.·cm^(-2),and the survival rates were 52%,29%and 5%.The rougher the surface of the substrate,the more the number of zygotes attached.Therefore,during the restoration of macroalgae,algal reefs with large base surface roughness and grain concave points can be transplanted by combining with artificial adhesive.