宫颈炎症状态对HPV感染的影响研究

Study on the Influence of Cervicitis Status on HPV Infection

目的:探讨宫颈炎症状态对人乳头瘤病毒(human papilloma virus,HPV)感染的影响。方法:运用E6-c-Myc-P2A-E7-c-3Flag基因慢病毒分别感染对照组(未处理的细胞)及实验组[经脂多糖(1μg/mL)诱导24 h的细胞],观察宫颈炎症状态对Myc-E6、Flag-E7标签蛋白表达的影响。结果:与对照组比较,实验组中人白细胞介素-6(interleukin-6,IL-6)、肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)、IL-1β、IL-17A mRNA的表达显著升高,差异有统计学意义(均P<0.05)。E6-c-Myc-P2A-E7-c-3Flag基因慢病毒感染72 h后,实验组和对照组转染效率分别是(89.05±0.16)%和(79.30±1.97)%,差异有统计学意义(P<0.05);实验组中Myc-E6和Flag-E7标签蛋白表达水平比对照组显著增加,差异有统计学意义(均P<0.05)。结论:宫颈上皮细胞处于炎症状态下更易感染HPV。

Objective:To investigate the effect of cervicitis status on human papilloma virus(HPV)infection.Methods:The E6-c-Myc-P2A-E7-c-3Flag gene lentivirus was used to infect the control group(untreated human normal cervical epithelial cells)and the experimental group[human normal cervical epithelial cells induced with lipopolysaccharide(1μg/mL)for 24 h],respectively,and observe the effect of cervicitis status on the expression of Myc-E6 and Flag-E7 tagged proteins.Results:Compared with the control group,the expression of human interleukin-6(IL-6),tumor necrosis factor-α(TNF-α),IL-1β,and IL-17A mRNA was significantly increased in the experimental group,and the differences were statistically significant(both P<0.05).After 72 h of infection with the E6-c-Myc-P2A-E7-c-3Flag gene lentivirus,the transfection efficiency of the experimental group and the control group was(89.05±0.16)%and(79.30±1.97)%,respectively,with a statistically significant difference(P<0.05).The expression of Myc-E6 and Flag-E7 tagged proteins in the experimental group was significantly increased compared to the control group,and the difference was statistically significant(both P<0.05).Conclusions:Cervical epithelial cells are more susceptible to HPV infection when they are in an inflammatory state.