产细菌素乳酸菌LS-1的全基因组测序分析

Whole Genome Sequencing Analysis of Bacteriocin-Producing Lactic Acid Bacteria LS-1

前期研究从内蒙古手工奶酪中筛选出1株可拮抗金黄色葡萄球菌的乳酸菌Lacticaseibacillus saniviri(LS-1)。为探究LS-1的全基因组信息,挖掘其潜在细菌素编码基因簇,本试验对LS-1进行全基因组测序,对编码基因进行预测,并采用非冗余蛋白质(Nr)和基因本体(GO)数据库进行功能注释;利用BAGEL4数据库预测分析细菌素合成基因簇。结果显示,LS-1的基因组大小为2356714 bp,GC含量为47.87%。共预测到2316个编码基因,其编码基因总长度为2058534 bp,平均长度为888 bp。LS-1所分泌细菌素属于Ⅲ类细菌素中的Enterolysin A(EnlA),推测EnlA可能通过裂解细胞壁来实现抑菌作用。本试验通过全基因组测序对LS-1基因组进行全面分析,从基因水平阐明其细菌素编码基因簇,进一步明确了菌株LS-1所产细菌素具有作为新型替抗产品的潜力。

In previous research,a lactic acid bacterium Lacticaseibacillus saniviri(LS-1)capable of antagonizing Staphylococcus aureus was isolated from handmade cheese in Inner Mongolia.To explore the complete genome information of LS-1 and uncover potential bacteriocin-encoding gene clusters,this study conducted whole genome sequencing of LS-1,predicted coding genes,and annotated them using the non-redundant protein(Nr)and gene ontology(GO)databases.The BAGEL4 database was used to predict and analyze bacteriocin synthesis gene clusters.The results revealed that the genome size of LS-1 was 2356714 bp with a GC content of 47.87%.A total of 2316 coding genes were predicted,with a total length of 2058534 bp and an average length of 888 bp.The secreted bacteriocin from LS-1 belongs to the Enterolysin A(EnlA)of Class III bacteriocins,suggesting that EnlA might exert bactericidal activity by lysing the cell wall.This study comprehensively analyzed the LS-1 genome through whole-genome sequencing,elucidating the bacteriocin-encoding gene clusters at the genetic level,and further confirming the potential of LS-1-produced bacteriocin as a novel antimicrobial product.