沉香叶化学成分研究

Study on the Chemical Composition the Leaves of Aquilaria sinensis

目的:研究沉香叶的化学成分,为其开发利用提供参考。方法:采用70%乙醇对沉香叶粉末进行回流提取,得到沉香叶浸膏。再经过高效液相色谱、凝胶柱层析、硅胶柱层析等分离手段对沉香叶醇提物进行提取分离,并通过质谱、核磁共振谱图对分离得到的化合物进行结构鉴定。结果:从沉香叶醇取物中分离得到18个化合物,分别为homostigmast-5-en-3β-ol(1)、表木栓醇(2)、木栓酮(3)、wallenone(4)、cyclobalanone(5)、羟基何帕酮(6)、β-谷甾醇(7)、3’,5-二羟基-7,4’-二甲氧基黄酮(8)、木犀草素-7,3’,4’-三甲醚(9)、5,4’-二羟基-7,3’-二甲氧基黄酮(10)、芫花素(11)、5,7-二甲氧基-4’-羟基二氢黄酮(12)、洋芹素-7,4’-二甲醚(13)、4-羟基-5,7-二甲氧基黄酮(14)、丁香脂素(15)、2,6-二甲氧基对苯醌(16)、2,5-二甲氧基对苯醌(17)、1,3,5-三甲氧基苯(18)。结论:从沉香叶醇提物中分离得到的18个化合物均为已知化合物,主要是黄酮和三萜类化合物,经文献查阅发现1、4、5、12、14化合物为首次从瑞香科植物沉香属中分离得到的。

Objective Through the study of chemical composition the leaves of Aquilaria sinensis.Methods The powdered the leaves of Aquilaria sinensis were extracted by using 70%ethanol。Various separation and purification methods such as HPLC was used to separate the chemical components of the leaves of Aquilaria sinensis,combined with 1H-NMR,13C-NMR and other spectroscopic techniques,and to analyze the structure of the obtained compounds for identification.Results eighteen compounds were obtained and identified as homostigmast-5-en-3β-ol(1),epifriedelanol(2),friedelin(3),wallenone(4),cyclobalanone(5),hydroxyhopanone(6),β-sitosterol(7),3’,5-Hydroxy-7,-4’-trimethoxyflavone(8),5-Hydroxy-7,-3’,4’-trimethoxyflavone(9),5,4’-Hydroxy-7,-3’-trimethoxyflavone(10),Genkwanin(11),5,7-Trimethoxy-4’-hydroxydihydroflavone(12),5-Hydroxy-7,4’-dimethoxyflavone(13),Apigenin 5,7-dimethyl ether(14),Syringareainol(15),2,6-dimethoxy-ρ-benzoquinone(16),2,5-dimethoxy-ρ-benzoquinone(17),1,3,5-trimethoxybenzene(18).Conclusion Compounds 1,4,5,12,and 14 were isolated from the leaves of Aquilaria sinensis for the first time.