外周血miR-326和miR-532-3p预测妊娠期糖尿病发病风险的潜在价值

Potential value of peripheral blood miR⁃326 and miR⁃532⁃3p in predicting the risk of developing gestational diabetes mellitus

目的 探讨外周血微小核糖核酸(miR)miR-326和miR-532-3p作为生物标记物在预测妊娠期糖尿病发病风险中的潜在价值。方法 选取2021年6月30日至2022年6月30日在莆田市第一医院登记的5例患病孕妇和5名健康孕妇作为患者组和对照组,收集、分析基线资料,并进行外周血转录组测序。同时,结合已发表数据集对miR进行筛选。采用皮尔逊相关系数法分析所获得的miR-326、miR-532-3p表达量与基线资料的相关性,并使用受试者工作曲线分析评估其预测价值。结果 患者组和对照组的餐后1小时血糖值差异有统计学意义(P<0.05)。测序数据表明miR-326(P<0.05)和miR-532-3p(P<0.05)的表达差异有统计学意义,且两者表达量呈显著正相关(r=0.87,P<0.05),该表达量与餐后1小时血糖均呈正相关(r=0.71、0.70,P均<0.05)。此外,餐后2小时血糖值与年龄(r=0.64,P<0.05)呈正相关,而胆固醇与甘油三酯水平(r=0.68,P<0.05)也呈正相关。miR-326与miR-532-3p在妊娠期糖尿病诊断中的曲线下面积均为0.96,联合检测可实现100%的灵敏度、80%的特异性和80%的准确性,对于预测妊娠期糖尿病发病风险具有较高的潜力。结论 miR-326和miR-532-3p的异常表达在预测妊娠期糖尿病的发病风险方面具有重要价值。

Objective To investigate the potential value of peripheral blood miR-326 and miR-532-3p as biomarkers for predicting the risk of gestational diabetes mellitus(GDM).Methods A total of 5 GDM patients and 5 healthy pregnant women registered at Putian First Hospital from June 30,2021 to June 30,2022 were selected and divided into the patient and the control group.Baseline data were collected and analysed,and transcriptome sequencing was conducted on peripheral blood samples.Moreover,miRs were screened using a combined publicly available dataset.Pearsons correlation analysis was used to assess the relationship between the expression levels of miR-326 and miR-532-3p and baseline data.The receiver operating curve(ROC)were constructed to evaluate the predictive value of miR-326 and miR-532-3p.Results There was a significant difference in postprandial blood glucose levels at 1 hour between the patient and control groups(P<0.05).The sequencing data showed statistically significant differential expression of miR-326(P<0.05)and miR-532-3p(P<0.05),and there was a significant positive correlation between their expression levels(r=0.87,P<0.05).Both miR-326 and miR-532-3p were positively correlated with postprandial blood glucose levels at 1 hour(r=0.71 and 0.70,P<0.05,respectively).In addition,postprandial blood glucose levels at 2 hours were positively correlated with age(r=0.64,P<0.05),and cholesterol levels were positively correlated with triglyceride levels(r=0.68,P<0.05).The area under the curve(AUC)for miR-326 and miR-532-3p in GDM diagnosis was 0.96.Furthermore,the combined detection of miR-326 and miR-532-3p achieved a sensitivity of 100%,specificity of 80%,and accuracy of 80%for diagnosing GDM,which has high potential for predicting the risk of GDM.Conclusion Aberrant expression of miR-326 and miR-532-3p is of significant value in predicting the risk of developing GDM during pregnancy.