响应面法优化菊花类黄酮微波辅助提取工艺

Optimization of Microwave-assisted Extraction of Flavonoids From Chrysanthemum by Response Surface Methodology

以‘W2’菊花为试材,采用微波辅助提取工艺,以菊花中总类黄酮提取率和7种类黄酮平均提取率为考察指标,使用中心组合试验设计、响应面分析法及Design Expert软件优化提取工艺参数,研究了微波辅助提取菊花中类黄酮成分的工艺,以期为进一步开发利用菊花资源提供参考依据。结果表明:最佳提取条件为溶剂70%乙醇(v/v)、提取时间75 s、微波功率80%、料液比1∶30 g·mL^(-1)。在该条件下,菊花中总类黄酮提取率和7种类黄酮平均提取率分别为91.20%和88.42%,与预测值91.26%和90.92%无显著差异。响应面分析法适于优化菊花类黄酮微波辅助提取工艺,试验结果可为菊花类黄酮工业化生产提供参考依据。

Taking‘W2’chrysanthemum as test materials,the microwave assisted extraction process was used,with the total flavonoids extraction rate and the average extraction rate of 7 flavonoids in chrysanthemum as the evaluation indicators.The extraction process parameters were optimized using central combination experimental design,response surface analysis,and Design Expert software.The microwave assisted extraction process of flavonoids in chrysanthemum was studied,in order to provide reference for further development and utilization of chrysanthemum resources.The results showed that the optimal extraction conditions were as follows,extraction solvent 70%ethanol(v/v),extraction time 75 seconds,microwave power 80%,solid-liquid ratio 1∶30 g·mL^(-1).Under these conditions,the extraction rate of total flavonoids and mean extraction rate of 7 flavonoids in‘W2’chrysanthemum were 91.20%and 88.42%,respectively,which were insignificantly different from those of predicted values 91.26%and 90.92%.RSM was suitable for MAE process optimization of flavonoids from chrysanthemum,and the results could provide reference for industrial production of chrysanthemum flavonoids.