益脑通络颗粒对pMCAO模型大鼠SOD、MDA及NO水平的影响

Effects of Yinao Tongluo Granule on Levels of SOD, MDA andNO in pMCAO Model Rats

目的:观察益脑通络颗粒对pMCAO模型大鼠血清SOD、MDA、NO的影响。方法:将大鼠随机分为假手术组、模型组、益脑通络颗粒低、中、高剂量组以及银杏叶组,每日1次,连续7 d,给药1周后采用线栓法制备pMCAO大鼠模型,24 h后采用Longa评分法对各组大鼠进行神经功能评分,干湿比重法测定各组大鼠脑组织含水量;TTC染色法测定各组大鼠脑梗死体积;TBA法检测各组大鼠血浆中MDA水平;羟胺法检测各组大鼠血清中SOD水平;酶法检测各组大鼠血清中NO含量。结果:1.与假手术组相比较,模型组大鼠神经功能评分及脑组织含水量明显升高,差异有统计学意义(P<0.05);与模型组比较,益脑通络颗粒高、中剂量和银杏叶组神经功能评分及脑组织含水量明显降低(P<0.05)。2.与假手术组相比较,模型组大鼠脑梗死体积明显减少(P<0.05);与模型组相比,银杏叶组及益脑通络颗粒低、中、高剂量组中的脑梗死体积均减少(P<0.05);与银杏叶组相比,益脑通络颗粒高剂量组的脑梗死体积明显减少(P<0.05)。3.与假手术组相比,模型组大鼠SOD含量明显下降、MDA和NO含量均明显升高(P<0.05);与模型组相比,银杏叶组和益脑通络颗粒高、中剂量组大鼠SOD含量明显升高、MDA和NO含量均明显下降(P<0.05);与银杏叶组相比,益脑通络颗粒高剂量组大鼠SOD含量明显升高、MDA和NO含量均明显下降(P<0.05)。结论:益脑通络颗粒可降低pMCAO模型大鼠脑梗死体积和脑组织含水量,提高pMCAO模型大鼠血清中SOD活性,降低MDA和NO水平,提示益脑通络颗粒可通过抑制氧化应激反应,从而起到保护神经元和减轻脑缺血损伤的作用。

Objective:To observe the effects of Yinao Tongluo Granule on serum levels of SOD,MDA and NO in pMCAO model rats.Methods:The rats were randomly divided into the sham operation group,the model group,the Yinao Tongluo Granule groups(low-dose,medium-dose and high-dose)and the ginkgo biloba group.The intervention was once per day for seven days.One week after intervention,suture-occluded method was applied to establish pMCAO model in rats;24 h after modeling,the Longa scoring method was adopted to evaluate nerve function score;water content was determined by dry/wet weight determination;TTC staining was used to determine the volume of cerebral infarction;TBA method was used to detect the plasma level of MDA;the serum level of SOD was detected by hydroxylamine method;the serum content of NO was detected by enzymic method.Results:Compared with those in the sham operation group,the neurological function score and the brain water content of the model group were significantly increased(P<0.05);compared with those in the model group,the neurological function score and the brain tissue water content were significantly decreased in the Yinao Tongluo Granule groups of medium-dose and high-dose,as well as in the Ginkgo biloba group(P<0.05).Compared with that in the sham operation group,the cerebral infarction volume was significantly increased in the model group(P<0.05);compared with that in the model group,the cerebral infarction volume was significantly decreased in the ginkgo biloba group and the Yinao Tongluo Granule groups of low-dose,medium-dose and high-dose(P<0.05);the cerebral infarction volume was less in the high-dose group than that in the ginkgo biloba group(P<0.05).Compared with those in the sham operation group,the SOD content was significantly decreased,the contents of MDA and NO were significantly increased in the model group(P<0.05);compared with those in the model group,the SOD content was significantly increased,and the contents of MDA and NO were significantly decreased in the ginkgo biloba group and the Yinao Tongluo Granule groups of medium-dose and high-dose(P<0.05);the SOD activity was higher and the levels of MDA and NO were lower in the high-dose group than those in the ginkgo biloba group(P<0.05).Conclusion:Yinao Tongluo Granule can reduce cerebral infarction volume and brain tissue water content in pMCAO model rats,it can increase SOD activity and decrease the levels of MDA and NO of pMCAO model rats,suggesting that Yinao Tongluo Granule can protect neurons and alleviate cerebral ischemia injury by inhibiting oxidative stress response.