上皮性卵巢癌HMGB3表达及预后价值的生物信息学分析

Analysis of HMGB3 expression and prognostic value in epithelial ovarian cancer based on bioinformatics

目的 探讨高迁移率族蛋白B3(HMGB3)在上皮性卵巢癌(EOC)中的表达及其与EOC患者预后的关系。方法 应用GEPIA2、UALCAN及TIMER 2.0数据库分析HMGB3在EOC组织中的表达水平及其与预后的相关性;通过UALCAN分析HMGB3可能参与调控的信号通路;利用TIMER 2.0分析HMGB3表达对肿瘤微环境中免疫细胞浸润的影响,分析HMGB3基因与PARP1、PARP2基因表达的相关性。结果 1)EOC组织中HMGB3的表达水平显著上调(P<0.05);2)HMGB3高表达EOC患者的无病生存率、总生存率均显著优于HMGB3低表达患者(P均<0.05);3)HMGB3高表达与CD4^(+)T细胞、CD8^(+)T细胞的高丰度相关(r=0.191,0.153,P均<0.05);4)HMGB3基因与PARP1、PARP2基因表达呈正相关(r=0.308,0.398,P均<0.001)。结论 HMGB3在EOC组织中显著上调,其高表达预示EOC患者预后良好,并与PARP1、PARP2基因表达相关,可作为EOC预后相关的潜在生物标志物。

Objective To explore the expression of high mobility group box B3(HMGB3)in epithelial ovarian cancer(EOC)and its relationship with the prognosis of EOC patients.Methods GEPIA2,UALCAN and TIMER 2.0 databases were used to analyze the expression level of HMGB3 in EOC tissues,its correlation with EOC patients?prognosis,the signaling pathways that HMGB3 may participate in regulation,the effects of HMGB3 expression on immune cell infiltration in tumor microenvironment,and the correlations between HMGB3 and PARP1/2.Results The expression levels of HMGB3 in EOC tissues were significantly upregulated(P<0.05).Disease-free survival and overall survival of EOC patients with high expression levels of HMGB3 were significantly higher than those with low expression levels of HMGB3(P<0.05).High expression levels of HMGB3 were correlated with the high abundance of CD4^(+)T cells and CD8^(+)T cells[correlation coefficients(r)were 0.191 and 0.153,respectively,both P<0.05].HMGB3 was positively correlated with the PARP1 and PARP2 genes(r=0.308,P<0.001;r=0.398,P<0.001).Conclusion HMGB3 is significantly upregulated in EOC tissues.High expression levels of HMGB3 predicts a good prognosis in EOC patients and is associated with the expression levels of PARP1 and PARP2 genes,which can serve as a potential biomarker for EOC patients.