WD重复结构域5B基因rs1067多态性与缺血性脑卒中不同证候及其血糖、血脂的关联

Correlation between WD repeat domain 5B gene rs1067 polymorphism with different syndromes of ischemic stroke,blood glucose and blood lipid

目的探讨WD重复结构域(WDR)5B基因rs1067多态性与缺血性脑卒中(IS)中医证候及临床标志物的关联性。方法选择IS患者及同期健康对照共1567例。WDR5B基因rs1067多态性的基因分型运用MassARRAY SNP基因分型方法。依据《中风病辨证诊断标准》对IS患者进行中医辨证分型。结果WDR5B基因rs1067多态性与IS风证[等位(A/G):P=0.037;显性(AA+GA/GG):P=0.043;加性(AA/GG):P=0.041]发生风险的关联性具有统计学意义;调整性别、年龄后,WDR5B基因rs1067多态性与IS风证[显性(AA+GA/GG):P=0.032;加性(AA/GG):P=0.032]发生风险显著相关。调整性别、年龄后,WDR5B基因rs1067多态性与IS患者的餐后2 h血糖、高密度脂蛋白水平具有统计学差异(P<0.05)。结论WDR5B基因rs1067多态性可能影响IS风证的发生风险,且WDR5B基因rs1067多态性可能影响IS患者餐后2 h血糖及高密度脂蛋白水平。

Objective To investigate the relationship between rs1067 polymorphism of WD repeat domain(WDR)5B gene and traditional Chinese medicine(TCM)syndromes and clinical markers of ischemic stroke(IS).Methods A total of 1567 patients with IS and healthy controls at the same time were selected.The genotyping of WDR5B gene rs1067 polymorphism was primarily performed with the sequenom MassARRAY SNP technology.The TCM syndromes of IS patients were classified according to the TCM Syndrome Differentiation Diagnostic Criteria for Apoplexy.Results WDR5B gene rs1067 polymorphism was significantly associated with the occurrence of wind syndrome of IS[allelic(A/G):P=0.037;dominant(AA+GA/GG):P=0.043;additive(AA/GG):P=0.041].After adjusting for sex and age,WDR5B gene rs1067 polymorphism was still significantly associated with the risk of IS syndrome[dominant(AA+GA/GG):P=0.032;additive(AA/GG):P=0.032].After adjusting for sex and age,WDR5B gene rs1067 polymorphism was significantly associated with postprandial 2 h plasma glucose level and high-density lipoprotein level in patients with IS(P<0.05).Conclusions WDR5B gene rs1067 polymorphism might affect the occurrence of wind syndrome of IS.WDR5B rs1067 gene polymorphism might affect postprandial 2 h plasma glucose level and high-density lipoprotein level in IS patients.