喀什地区维吾尔族妊娠妇女MTHFR C677T基因突变、血清Hcy、叶酸及维生素B_(12)水平与自然流产的关系

Relationship between MTHFR C677T gene mutation,and the levels of serum Hcy,folic acid,and vitamin B_(12)with spontaneous abortion in Uygur pregnant women in Kashgar area

目的探讨喀什地区维吾尔族妊娠妇女MTHFR C677T基因突变、血清同型半胱氨酸(Hcy)、叶酸及维生素B_(12)水平与自然流产的关系。方法选取2020年1月至2021年6月新疆维吾尔自治区喀什地区第一人民医院(以下简称“我院”)生殖医学中心就诊的75例自然流产患者为观察组,另选取同期我院体检的75名健康孕妇为对照组。检测两组MTHFR C677T位点基因多态性,血清Hcy、叶酸、维生素B_(12)水平;比较不同MTHFR C677T基因型、血清Hcy、叶酸、维生素B_(12)水平;分析喀什地区维吾尔族妊娠妇女自然流产的影响因素。结果观察组人工流产史、孕早期感染占比及人工流产次数大于对照组(P<0.05)。两组基因型、等位基因分布比较,差异有统计学意义(P<0.05)。观察组血清Hcy水平高于对照组,叶酸及维生素B_(12)水平低于对照组(P<0.05)。TT基因型患者血清Hcy高于CT、CC基因型,叶酸及维生素B_(12)水平低于CT和CC基因型(P<0.05);CT和CC基因型患者血清Hcy、叶酸及维生素B_(12)水平比较,差异无统计学意义(P>0.05)。TT基因型、高Hcy水平是喀什地区维吾尔族妊娠妇女自然流产的危险因素(OR=2.044、2.010,P<0.05),高叶酸和维生素B_(12)水平是喀什地区维吾尔族妊娠妇女自然流产的保护因素(OR=0.587、0.614,P<0.05)。结论MTHFR C677T基因型,血清Hcy、叶酸、维生素B_(12)水平与喀什地区维吾尔族妊娠妇女自然流产有关。

Objective To investigate the relationship between MTHFR C677T gene mutation,and the levels of serum homocysteine(Hcy),folic acid,and vitamin B_(12)with spontaneous abortion in Uygur pregnant women in Kashgar area.Methods From January 2020 to June 2021,75 spontaneous abortion patients in the Reproductive Medicine Center of the First People’s Hospital of Kashi,Xinjiang Uygur Autonomous Region(hereinafter referred to as“our hospital”)were selected as the observation group,and 75 healthy pregnant women who underwent physical examination in our hospital during the same period were selected as the control group.The polymorphism of MTHFR C677T locus gene and the levels of serum Hcy,folic acid,and vitamin B_(12)between two groups were detected;the levels of serum Hcy,folic acid,and vitamin B_(12)levels among different MTHFR genotypes were compared;the influencing factors of spontaneous abortion among Uyghur pregnant women in Kashgar area were analyzed.Results The proportion of induced abortion history,infection in early pregnancy,and the number of induced abortion in the observation group were higher than those in the control group(P<0.05).There were significant differences in genotype and allele distribution between the two groups(P<0.05).The level of serum Hcy in the observation group was higher than that in the control group,and the levels of folic acid and vitamin B_(12)were lower than those in the control group(P<0.05).The level of serum Hcy of TT genotype was higher than that of CT and CC genotype,and the levels of folate and vitamin B_(12)were lower than those of CT and CC genotype(P<0.05);there were no significant differences in the levels of serum Hcy,folic acid,and vitamin B_(12)between CT and CC genotype patients(P>0.05).TT genotype and high Hcy level were risk factors for spontaneous abortion in Uyghur pregnant women in Kashgar area(OR=2.044,2.010,P<0.05),and high folic acid and vitamin B_(12)levels were protective factors for spontaneous abortion in Uyghur pregnant women in Kashgar area(OR=0.587,0.614,P<0.05).Conclusion MTHFR C677T genotype,the levels of serum Hcy,folic acid,and vitamin B_(12)are associated with spontaneous abortion in Uyghur pregnant women in Kashgar area.