豚鼠被动皮肤过敏试验条件研究

Study on the Conditions of Passive Cutaneous Anaphylaxis Test in Guinea Pigs

目的探讨豚鼠被动皮肤过敏(PCA)试验的最佳条件(致敏阳性物质、时间)。方法采用卵白蛋白(ova,0.4%)和牛血清白蛋白(BSA,1%)为PCA抗原,分别于不同激发时间(4 h和24 h)对不同生产厂家(潍坊圣诺实验动物养殖有限公司、青岛康大生物科技有限公司、济南金丰实验动物有限公司,简称圣诺、康大、金丰)的豚鼠进行PCA试验,记录蓝斑直径。结果在ova被动致敏4 h后,3个生产厂家的豚鼠产生的蓝斑分界清晰且直径均大于5.0 mm(阳性);在ova被动致敏24 h后,3个生产厂家的豚鼠产生的蓝斑已混在一起、分界不清,无法测量。在BSA被动致敏4 h后,圣诺和康大生产的豚鼠产生的蓝斑分界清晰且直径均大于5.0 mm,金丰生产的豚鼠仅含抗体血清(1∶2、1∶4,V/V)的蓝斑直径大于5.0 mm;在BSA被动致敏24 h后,金丰生产的豚鼠的蓝斑分界清晰且直径大于5.0 mm,圣诺和康大生产的豚鼠产生的蓝斑已混在一起、分界不清,无法测量。结论豚鼠PCA中首选0.4%ova溶液作为试验抗原时致敏时间宜为4 h。

Objective To investigate the optimal conditions(allergenic positive substance and time)of passive cutaneous anaphylaxis(PCA)test in guinea pigs.Methods Ovalbumin(ova,0.4%)and bovine serum albumin(BSA,1%)were used as the PCA antigens,the PCA test was performed on guinea pigs from different manufacturers(Weifang Shengnuo Experimental Animal Breeding Co.,Ltd.,Qingdao Kangda Biological Technology Co.,Ltd.,Jinan Jinfeng Experimental Animal Co.,Ltd.,hereinafter referred to as the Shengnuo,Kangda and Jinfeng respectively)at different sensitization time(4 h and 24 h),and the diameter of the blue spots was recorded.Results After the passive sensitization with ova for 4 h,the blue spots of guinea pigs from three manufacturers had clear boundaries,and the diameters were greater than 5.0 mm(positive);while after the passive sensitization with ova for 24 h,the above blue spots were mixed,with unclear boundaries and unable to be measured.After passive sensitization with BSA for 4 h,the blue spots of guinea pigs from Shengnuo and Kangda had clear boundaries,and the diameters were greater than 5.0 mm,while the blue spots of guinea pigs from Jinfeng sensitized by only serum containing antibodies(1∶2,1∶4,V/V)had a diameter greater than 5.0 mm.After passive sensitization with BSA for 24 h,the blue spots of guinea pigs from Jinfeng had clear boundaries,and the diameters were greater than 5.0 mm,while the blue spots of guinea pigs from Shengnuo and Kangda were mixed and indistinguishable,which were unable to be measured.Conclusion In PCA test of guinea pigs,0.4%ova solution is preferred as the test antigen,and the sensitization time of 4 h is suitable.