外源抗菌肽对木荷体内微生物和代谢物的影响

Effects of Exogenous Application of Antimicrobial Peptides on Microorganisms andMetabolites in Schima superba

抗菌肽是植物防御病原体的重要天然组成部分,是高效、高通量、生态友好的抗病手段。为改善木荷(Schima superba Gardn.et Champ.)人工林的林分质量,验证抗菌肽对木荷内环境的影响,于2021年对福建省泰宁国有林场2年生木荷幼苗叶片喷洒500倍抗菌肽双蒸水稀溶液(以叶片喷洒双蒸水作为对照),对其微生物与代谢物内环境进行分析。结果表明:施用抗菌肽后木荷体内的优势菌目:肠杆菌目、伯克氏菌目、生丝微菌目、红细菌目、芽孢杆菌目以及球囊霉目丰度显著(P≤0.05)升高,说明施用抗菌肽显著提高木荷的抗逆性,有助于木荷的生长发育。共筛选到687个差异代谢物,其中上调的差异代谢物有233个,占比34%;下调的差异代谢物有454个,占比66%。主要富集在ABC转运蛋白(ABC transporters)通路、花生四烯酸代谢(Arachidonic acid metabolism)通路、类黄酮生物合成(Flavonoid biosynthesis)通路、苯丙烷类生物合成(Phenylpropanoid biosynthesis)通路。综合结果表明,添加抗菌肽有助于提高木荷体内的能量和物质代谢,提高木荷应答逆境胁迫的能力。本研究结果可为利用抗菌肽防治木荷病害,提高林分质量及持续经营木荷人工林的生产实践提供参考。

Antimicrobial peptides are important natural components of plant de fense against pathogens and are efficient,high-throughput and eco-friendly means of disease resistance.In order to improve the stand quality of Schim a superba plantation forests and to verify the effect of antimicrobial p eptides on the i nternal environment of S.superba the internal environment of microorganisms and metabolites of 2-year-old S.superba seedlings was tr eated by spraying their leaves with a dilute 500-fold antimicrobial peptide bi-distillate soluti on in 2021 in the state-owned forest farms of Taining,Fujian Province(leaf spra y ing with bi-distillate as a control),and the internal environment of microorga n isms and metabolites was the internal environment of microorganisms and metaboli tes was analyzed.The results showed that the abundance of Enterobacteriaceae,Burkholderiaceae,Microflora,Erythrobacteriaceae,Bacillariophyta and Mycobacte rium globosum increased significantly(P≤0.05)after the appli cation of anti microbial peptide.It indicated that the application of antimicrobial peptides significantly improved the stress tolerance of S.superba a nd contributed to the growth and development of S.superba.A total of 687 diffe rential me tabolites were screened,including 233 up-regulated differential metabolites,accounting for 34%,and 454 down-regulated differential metabolites,accounting for 66%.They were mainly enriched in the ABC transporters pathway,the arachidon icacid metabolism pathway,the flavonoid biosynthesis pathway,the phenylpropan oid biosynthesis pathway.This indicates that addition of ant imicrobial peptides can help to improve the energy and material metabolism in S.superba,and enhance the ability of S.superbato respond to adversity stress.The results of this study can provide a reference for the production practice of using antimicrobial peptides to prevent and control the diseases of S.superba to improve the quality of forest stands and to sustain the management of S.superba plantation forests.