子宫内膜癌组织中lncRNA XIST、miR-132-3p的表达及与病理参数和预后的关系

Expression of lncRNA XIST and miR-132-3p in endometrial cancer tissues and the relationship with pathological parameters and prognosis

目的探讨子宫内膜癌(EC)组织中长链非编码RNA X非活性特异性转录本(lncRNA XIST)、微RNA-132-3p(miR-132-3p)的表达及与病理参数和预后的关系。方法选取2016年2月至2019年12月江苏省南通市第六人民医院及江苏省南通市肿瘤医院收治的100例EC患者,采用q PCR检测癌组织与癌旁正常组织中lncRNA XIST、miR-132-3p表达。Star Base数据库预测lncRNA XIST与miR-132-3p的关系,Pearson相关性分析lncRNA XIST与miR-132-3p的相关性。随访3年,根据EC组织中lncRNA XIST、miR-132-3p的平均表达量将患者分为高表达组和低表达组,采用K-M法绘制不同lncRNA XIST、miR-132-3p表达患者的生存曲线。结果癌组织中lncRNA XIST表达高于癌旁正常组织,miR-132-3p表达低于癌旁正常组织(P<0.05)。lncRNA XIST与miR-132-3p存在互补序列,lncRNA XIST与miR-132-3p表达呈负相关(r=-0.694,P<0.01)。不同分化程度、国际妇产科联盟(FIGO)分期、脉管侵犯和淋巴结转移EC组织中lncRNA XIST、miR-132-3p表达比较,差异有统计学意义(P<0.05)。lncRNA XIST高表达组3年累积生存率低于lncRNA XIST低表达组,miR-132-3p高表达组3年累积生存率高于miR-132-3p低表达组(P<0.05)。结论EC组织中lncRNA XIST高表达和miR-132-3p低表达,两者与分化程度、FIGO分期、脉管侵犯、淋巴结转移及预后有关。

Objective To investigate the expression of long non-coding RNA X inactive specific transcript(lncRNA XIST),microRNA-132-3p(miR-132-3p)in endometrial cancer(EC)tissues and the relationship with pathological parameters and prognosis.Methods A total of 100 EC patients admitted to the Sixth People’s Hospital of Nantong and Nantong Tumor Hospital,Jiangsu Province from February 2016 to December 2019 were selected.qPCR was used to detect the expression of lncRNA XIST and miR-132-3p in cancer and paracancer tissues.The StarBase database predicted the relationship between lncRNA XIST and miR-132-3p,and Pearson correlation analysis was used to correlate the expression of lncRNA XIST and miR-132-3p.At three years follow-up,according to the average expression of lncRNA XIST and miR-132-3p in EC tissues,the patients were divided into high expression group and low expression group,and the survival curves of patients with different lncRNA XIST and miR-132-3p expression in EC tissues were plotted using the K-M method.Results lncRNA XIST expression in cancer tissue was higher than that in paracancerous normal tissue,and miR-132-3p expression was lower than that in paracancerous normal tissue(P<0.05).There was a complementary sequence between lncRNA XIST and miR-132-3p,lncRNA XIST was negatively correlated with miR-132-3p expression in EC tissues(r=-0.694,P<0.01).The expression of lncRNA XIST and miR-132-3p in EC tissues of different differentiation degrees,International Federation of Obstetrics and Gynecology(FIGO)stage,vascular invasion,and lymph node metastasis were statistically significant(P<0.05).The 3-year cumulative survival rate of the lncRNA XIST high expression group was lower than that of the lncRNA XIST low expression group,and the 3-year cumulative survival rate of the miR-132-3p high expression group was higher than that of the miR-132-3p low expression group(P<0.05).Conclusion The expression of lncRNA XIST is high and that of miR-132-3p is low in EC tissue,and its correlate with differentiation degree,FIGO stage,vascular invasion,and lymph node metastasis and prognosis.