复合多肽延缓小鼠皮肤衰老的功效研究

Effects of compositions of bioactive peptides on skin aging in mice

通过细胞增殖、抗氧化、抗炎以及促进胶原蛋白4个细胞实验研究了棕榈酰三肽-5,五肽-3,大豆多肽,类蛇毒肽和六肽-115种多肽的抗衰能力和复合后的效果提升作用,采用UVB联合D-半乳糖皮下注射造模方式研究了5种多肽复合后的皮肤抗衰老作用。结果表明,5种多肽具有一定的促细胞增殖、抗氧化、抗炎及促胶原蛋白合成能力,且5种多肽复合后具有显著的效果提升作用,复合多肽能够显著改善和缓解UVB联合D-半乳糖皮下注射引起的小鼠皮肤表皮增厚、胶原较少、弹性蛋白减少情况,并且能够显著抑制造模引起的IL-1,IL-6,TNF-α表达上调,抑制MMP3,MMP9,β-半乳糖苷酶的表达。以上结果表明5种多肽复合能够通过缓解UVB联合D-半乳糖皮下注射造模造成的表皮增厚、炎症、细胞外基质流失及衰老相关酶表达增加来发挥皮肤抗衰老作用。

Delaying skin aging has always been a hot topic in cosmetic research.In recent years,it has been reported that active peptides have the effect of delaying skin aging,thus research on substances that delaying skin aging is of great significance to the development of cosmetics and the application in the field of skin treatment.In the present study,we evaluated the anti-aging abilities of five bioactive peptides and the enhancing effect of the poly-peptide composition containing five bioactive peptides by four cell experiments and Elisa assay of cell proliferation,anti-oxidation,antiinflammation,and promotion of collagen.Then,UVB radiation combined with D-galactose subcutaneous injection was adopted to establish animal skin aging model to investigate the anti-aging effects of the poly-peptide composition.Our data demonstrates that the five bioactive peptides have certain ability to promote cell proliferation,anti-oxidation,antiinflammatory and promote collagen synthesis,and the poly-peptide composition have a significant enhancing effect in vitro.Importantly,after the administration of poly-peptide composition in vivo,the epidermal thickness decreases and tends to restore to normal(P<0.05),and the contents of collagen and elastin increase,respectively(P<0.05).Enzyme-linked immunosorbent assay results show that after the administration of poly-peptide composition,the expression of IL-1,IL-6,and TNF-αare down-regulated(P<0.05),the expression of MMP3 and MMP9 is inhibited(P<0.05),and the increased expression ofβ-galactosidase is significantly reduced(P<0.05).The above results suggest that the composition of five bioactive peptide can play an anti-aging effect on the skin caused by UVB radiation combined with D-galactose subcutaneous injection.