基于转运蛋白工程提升微生物菌株耐受性和生物制造效率的研究进展

Research Progress in the Improvement of Microbial Strain Tolerance and Efficiency of Biological Manufacturing Based on Transporter Engineering

微生物细胞工厂广泛用于生物燃料以及高值化学品和大宗化学品的可持续生产,但是高浓度产物和底物以及多种环境胁迫条件会抑制菌株的发酵效率,降低生产的经济性。因此,增强菌株耐受性对于目的产物的高效和可持续生产至关重要。近年来,利用转运蛋白工程保护菌株免受毒性化合物的损害以提升菌株耐受性的策略日益受到研究者的关注。因此,本文总结了基于微生物转运蛋白工程改造提升菌株耐受性的研究进展,分析了目前微生物转运蛋白研究领域中存在的关键问题,并探讨了基于转运蛋白工程提升微生物菌株耐受性的策略,尤其对人工智能在转运蛋白功能注释、结构模拟和底物-转运蛋白互作预测中的应用进行了总结和展望,以期能够促进微生物在绿色生物制造领域的应用。

Microbial cell factories have been extensively used for sustainable production of biofuels,as well as high value and bulk chemicals.However,high concentration products or substrates,as well as stressful conditions during industrial production,may compromise fermentation efficiency and decreasing economics of production.In this context,microbial stress tolerance is crucial for green and sustainable production of the target products.In recent years,the use of transporters to protect microbial cells from toxic compounds for enhancing strain tolerance has received increasing worldwide attention.This review summarizes the progress of studies on microbial strain tolerance enhancement based on transporter engineering,analyses the current key points in the field of transporter research and discusses strategies to enhance strain tolerance based on transporter manipulation.Especially,the review highlights the applications of artificial intelligence in transporter annotation,structure simulation and substrate-transporter interaction prediction,aiming to promote the application of microorganisms in biological manufacturing.