ABA和干旱胁迫下菊花脑ZF-HD基因家族的表达分析

Expression Analysis of the ZF-HD Gene Family in Chrysanthemum nankingense Under Drought and ABA Treatment

干旱是影响菊花生长发育和产量的重要非生物因子,在应对干旱胁迫时ZF-HD转录因子家族起着重要的作用。菊花脑是研究菊花遗传育种机制的模式植物,筛选其抗旱相关基因可为菊花相关研究奠定基础。本研究在菊花脑基因组基础上,建立了本地数据库以实现本地BLAST,并利用Pfam数据库、HMMER、DNAMAN、MEGA、Tbtools、ExPASy、WOLF PSORT在线网站及实时荧光定量技术,对ZF-HD基因家族进行筛选以及生物信息学和表达分析。最终在菊花脑基因组中共鉴定出19个CnZFHD基因,分为ZHD、MIF两个亚家族,其中ZHD亚族包括I-VI亚群。家族成员的组织特异性表达具有时间和空间差异性,且启动子区富含与生长发育、胁迫和激素相关的响应元件。RT-qPCR结果显示,19个CnZF-HD基因均响应干旱胁迫和ABA处理,且在干旱条件下均被诱导表达。CnZF-HD4、CnZF-HD5、CnZF-HD9、CnZF-HD10、CnZF-HD14、CnZF-HD15、CnZF-HD19这7个基因在ABA处理中被诱导表达,亚群VI在调控菊花脑抗旱性方面起着重要作用。另外,CnZF-HD1基因的表达在干旱条件下持续上调,可作为抗旱相关的候选基因,但其对ABA的敏感性需要进一步研究。

Drought is an important abiotic factor affecting the growth and yield of chrysanthemum,while ZF-HD transcription factor family plays an important role in coping with drought stress.Chrysanthemum nankingense is a model plant for studying genetic and breeding mechanism of chrysanthemum,screening its drought resistance related genes may lay a foundation for the relevant research in chrysanthemum.In this study,we established a local database and achieving local BLAST based on the C.nankingense genome,and used the Pfam database,HMMER,DNAMAN,MEGA,Tbtools,ExPASy,WoLF PSORT online sites and real-time fluorescence quantification for screening as well as bioinformatics and expression analysis of ZF-HD family genes.The results showed that a total of 19 ZF-HD gene family members were identified in the C.nankingense,divided into two subfamilies,ZHD and MIF,with the ZHD subfamily including subgroup I-VI.The tissue specific expressions of family members were temporally and spatially variable,their promoter regions were enriched with growth and development,stress and hormone-related response elements.The results of RT-qPCR showed that 19 CnZF-HD genes all responded to the drought stress and ABA treatment,and expression was induced under drought conditions.The genes CnZF-HD4,CnZF-HD9,CnZF-HD10,CnZF-HD14,CnZF-HD15 and CnZF-HD19 were also induced to be expressed in ABA treatment.Subgroup VI played an important role in regulating the drought resistance of C.nankingense.In addition,the expression of CnZF-HD1 gene was continuously up-regulated under drought conditions,thus it could be a candidate gene for drought resistance,but its sensitivity to ABA needs further study.