雀舌草醇提物抗氧化与美白功效研究

为探究雀舌草的抗氧化和美白功效,进行多种自由基清除实验,透明质酸酶抑制实验和小鼠黑色素瘤细胞(B16F10)内黑色素量及酪氨酸酶活性实验,发现其醇提物对DPPH、ABTS+自由基清除率和铜离子螯合能力的IC50值分别为40.37±3.28、24.92±0.98和303.25±4.71μg/mL,铁和铜离子还原能力的EC50值分别为637.25±10.37和110.25±1.55μg/mL。浓度为1.33 mg/mL时对透明质酸酶抑制率为100%;在100μg/mL时,对细胞内黑色素量和酪氨酸酶活性都有显著抑制效果。由上述结果得知雀舌草醇提物有抗氧化和美白作用,有望应用于化妆品中。

To explore the antioxidant and whitening ability of ethanol extract from Stellaria alsine Grimm,this study conducted a variety of experiments,including free radical scavenging assay,hyaluronidase inhibition assay as well as intracellular melanin content and tyrosinase activity assay in B16F10 murine melanoma cells.The evaluation of DPPH and ABTS+free radical scavenging capability,and copper ion chelating ability,yield IC50 values of 40.37±3.28,24.92±0.98 and 303.25±4.71μg/mL,respectively.The EC50 values of ferric and cupric reducing antioxidant power were 37.25±10.37 and 110.25±1.55μg/mL,respectively.The 1.33 mg/mL ethanol extract had a 100%hyaluronidase inhibition rate.The 100μg/mL ethanol extract demonstrates significantly inhibitory effects on intracellular tyrosinase activity and melanin content.The experimental data verify the antioxidant and whitening efficacy of Stellaria alsine Grimm,which provides theoretical references for its further application in cosmetics.