两株分离自木塑复合材料的腐朽真菌的分离鉴定及其腐朽性能研究

Isolation and Identification of Two Decaying Fungi Isolated from Wood Plastic Composite Materials and Study on Their Decay Properties

从户外腐朽木塑复合材料中分离并纯化得到两株真菌,分别命名为WPC-11及WPC-12。通过对菌株的平板菌落、显微形态特征的观察,以及ITS序列和18S rDNA序列的分析,确认WPC-11属于白腐菌多年卧孔菌属(Perenniporia sp.)菌株,而WPC-12则属于褐腐菌拟层孔菌属(Fomitopsis sp.)菌株。为评估这两株真菌的腐朽性能,进行了木材天然耐腐性试验,并使用扫描电镜(SEM)及傅里叶红外光谱(FTIR)对腐朽前后木材试样的微观结构和化学成分的变化进行了分析。结果表明,WPC-11和WPC-12分别对黄瑾木材造成了39.06%及47.39%的质量损失。在腐朽后的SEM图像中可观察到木材试样的表面和内部均已被大量降解。而FTIR则表明,木材试样中的多种化学组分在受到腐朽真菌作用后发生了降解。

Two fungi,WPC-11 and WPC-12,were isolated and purified from decayed wood-plastic composites.Based on the characteristics of plate colony and micromorphology,as well as the analysis of ITS and 18S rDNA sequences,WPC-11 was identified as Perenniporia sp.species,while WPC-12 was identified as Fomitopsis sp.strain.The decay properties of two decay fungi were tested by method of natural wood decay resistance test.The changes of microstructure and chemical composition of wood samples before and after fungal decay were analyzed by scanning electron microscopy(SEM)and Fourier infrared spectroscopy(FTIR).The results showed that WPC-11 and WPC-12 had caused 39.06%and 47.39%weight losses for Hibiscus tiliaceus wood,respectively.SEM showed that the surface and interior of the wood specimens after decay had been degraded.Meanwhile,the FTIR results indicated that chemical components in wood samples have been degraded by decay fungi.