基于末端脱氧核苷酸转移酶协同G-四链体核酶的恩诺沙星检测方法

A method for enrofloxacin detection based on collaborative signal amplification by terminal deoxynucleotidyl transferase with G-quadruplex ribozyme

基于末端脱氧核苷酸转移酶(TdT)协同G-四链体核酶设计信号放大策略,建立了一种恩诺沙星电化学检测方法。目标物恩诺沙星与特异性核酸适体的结合触发TdT在电极表面的扩增反应,产生G-四链体核酶纳米线结构,进而发挥辣根过氧化物酶活性催化信号放大,实现恩诺沙星的高灵敏和高特异性检测。该方法对恩诺沙星的线性检测范围为0.5~50μg/L,检测限低至0.043μg/L。此外,该无标记电化学生物传感器简单快速,成本低,并成功应用于对实际食品样本的分析检测,显示出较好的应用潜能。

A electrochemical method for enrofloxacin detection was established based on the signal amplification strategy designed by collaborating terminal deoxynucleotidyl transferase(TdT)with G-quadruplex ribozyme.The binding of target enrofloxacin with specific aptamer triggered the extension reaction of TdT on the electrode surface,resulting in the formation of G-quadruplex ribozyme nanowires which mimicked horseradish peroxidase activity to catalyze signal amplification,and finally achieving highly sensitive and specific detection of enrofloxacin.The linear detection range of this method for enrofloxacin was 0.5~50μg/L with the detection limit as low as 0.043μg/L.In addition,the label free electrochemical biosensor is simple,fast and low cost,and has been successfully applied to the analysis of real food samples,showing good application potential.