浒苔多酚对2型糖尿病小鼠降血糖功效研究

Effect of Polyphenols from Enteromorpha prolifera on Reducing Blood Glucose in Type 2 Diabetic Mice

目的:为探究浒苔多酚(Enteromorpha prolifera polyphenols,EPP)对2型糖尿病小鼠的降血糖功效,为浒苔多酚产品的开发提供理论依据。方法:以浒苔为材料,经乙醇溶液提取得到EPP粗提物,采用硅胶柱层析纯化EPP粗提物;用四氧嘧啶诱导建立2型糖尿病小鼠模型,将实验小鼠分为空白对照组、模型对照组、EPP低剂量组(EPP-L)、EPP中剂量组(EPP-M)、EPP高剂量组(EPP-H)、阳性对照组,连续灌胃4周,测定小鼠基础指标,以及脂代谢和血清抗氧化指标。结果:在动物实验中,第4周时,EPP-H组小鼠体重达32.16±1.97 g,与阳性对照组无显著差异(P>0.05)。EPP-H组小鼠空腹血糖浓度为11.78±1.62 mmol/L,与阳性对照组无显著差异(P>0.05),且能显著提升小鼠葡萄糖耐受力(P<0.05),改善小鼠耐糖量异常。EPP-H组小鼠体内超氧化物歧化酶、过氧化氢酶、谷胱甘肽过氧化物酶、高密度脂蛋白胆固醇浓度及血清胰岛素水平分别为:157.36±6.71 U/mg,168.07±1.77 U/mg,378.14±9.74 U/mg,1.31±0.04 mmol/L,19.03±2.01 mU/L,与模型对照组相比极显著升高(P<0.01),且能显著降低丙二醛、甘油三酯、总胆固醇、低密度脂蛋白胆固醇的含量(P<0.05)。结论:浒苔多酚能有效改善2型糖尿病小鼠基础代谢情况及修复糖尿病小鼠的过氧化损伤,进而起到降血糖作用。

Objective:The aim of this work was to explore the hypoglycemic effect of Enteromorpha prolifera polyphenols(EPP)on type 2 diabetic mice,and to provide theoretical basis for the development of EPP products.Methods:EPP crude was extracted from Enteromorpha prolifera by ethanol solution extraction,and purified by silica gel column chromatography.The model of type 2 diabetes in mice was established by alloxan.The experimental mice were divided into blank control group,model control group,EPP low dose group(EPP-L),EPP midium dose group(EPP-M),EPP high dose group(EPP-H),and positive control group.The basic indicators of mice,as well as lipid metabolism and serum antioxidant indicators,were measured by continuous gastric perfusion for 4 weeks.Results:At the 4th week of the experiment,the averaged weight of mice in EPP-H group reached 32.16±1.97 g,which was not significantly different from that of the positive control group(P>0.05).The fasting blood glucose concentration of EPP-H group was 11.78±1.62 mmol/L,and there was no significant difference between EPP-H group and the positive control group(P>0.05).EPP-H clearly had significantly improved both the glucose tolerance(P<0.05)and the abnormal glucose tolerance of mice.The concentrations of superoxide dismutase,catalase,glutathione peroxidase,high-density lipoprotein cholesterol,and serum insulin levels in EPP-H mice were 157.36±6.71 U/mg,168.07±1.77 U/mg,378.14±9.74 U/mg,1.31±0.04 mmol/L,and 19.03±2.01 mU/L,respectively,compared with the model control group,it was significantly increased(P<0.01),and the contents of malondialdehyde,triglyceride,total cholesterol and low-density lipoprotein cholesterol were significantly decreased(P<0.05).Conclusion:Polyphenols from Enteromorpha prolifera can effectively improve the basal metabolism of type 2 diabetic mice and repair the peroxide damage of diabetic mice,and may play a role in lowering blood sugar.