基于质量编码探针的质谱多联检测研究进展

Advances in mass-encoded probes for multiplex mass spectrometric detec⁃tion

质量编码探针是一类以质量标签分子对待测物进行特异性识别标记并输出其特征离子信号的探针工具,其在疾病标志物多元分析和药物靶点筛选等方面都发挥着重要的作用。近年来,研究者基于各类质谱分析方法,开发了不同结构与功能的质量编码探针,为生理环境中生物分子的多重检测以及组织样本的质谱成像提供了有力的技术工具。本文从探针结构组成、质谱分析方法以及在生化分析中的应用3个方面综述了质量编码探针在质谱多联检测方面的最新研究进展,并对其未来的发展方向进行了展望。

Mass-encoded probe is a probing tool that specifically identifies target molecules and thus outputs their characteristic ion signals with mass tags.It plays an important role in multiplex assay of disease markers,drug target screening and other biomedical applications.Based on various mass spectrometric methods,researchers have developed an array of mass tag-encoded probes with different structures and functions,providing powerful technical tools for multiplex detection of biomolecules in physiological environments and for mass spectrometry imaging of tissue samples.This review introduces the latest research progress of mass tag-encoded probes in multiplex mass spectrometric detection from three aspects,i.e.structural composition of the probes,mass spectrometric methods and their application in biochemical analysis,with a prospect of the future development of mass tag-encoded probes.