萌发期耐莠去津苜蓿种质资源筛选及评价

Screening and Evaluation of the Tolerance of Atrazine to Alfalfa Germplasms Resources During Germination Period

为探讨苜蓿萌发期耐除草剂莠去津特性,分析莠去津胁迫下37份苜蓿品种的生长发育特性,建立其筛选的评价体系,筛选萌发期耐莠去津苜蓿种质,将莠去津耐性度量值(A值)、平均隶属函数值(AFV值)、加权关联度系数(WAC值)与相关性分析、主成分分析、聚类分析相结合,鉴定苜蓿材料萌发期对莠去津的耐性。结果表明,在21 mg/L的莠去津溶液胁迫下,37份苜蓿品种幼苗的根长、干重与SPAD值受抑制较为严重。根据A值、AFV值、WAC值的综合排序结果,对莠去津耐性较强的品种是要塞、菲力、驯鹿。A值聚类分析将供试品种分为4个耐性级别,其中Ⅰ级仅有1份(要塞),Ⅱ级13份,Ⅲ级9份,Ⅳ级14份。综合评价排序得出,对莠去津耐性最强的品种为要塞,其次为菲力、迪特、驯鹿,这几个品种可作为苜蓿耐莠去津育种和耐性机理研究的重要种质资源。

The study aimed to investigate the herbicide atrazine tolerance characteristics of 37 alfalfa varieties at the germination stage,analyse the growth and development characteristics of different alfalfa under atrazine stress and establish an evaluation system for its screening,and select alfalfa germplasms tolerant to atrazine at the germination stage.The atrazine tolerance measure(A value),average affiliation function value(AFV value)and weighted correlation coefficient(WAC value)were combined with correlation analysis,principal component analysis and cluster analysis to identify the atrazine tolerance of alfalfa materials at the germination stage.The results showed that root length,dry weight and SPAD of 37 alfalfa varieties were more severely inhibited under the stress of 21 mg/L atrazine solution concentration Base on A value,AFV value and WAC value comprehensive rankings,the varieties more tolerant to atrazine were Fortress,Philly and Reindeer.The A value clustering analysis classified the tested varieties into four tolerance classes,with only one variety of Fortress in ClassⅠ,13 varieties in ClassⅡ,9 varieties in ClassⅢand 14 varieties in ClassⅣ.The most atrazine-tolerant varieties were identified as Fortress,followed by Philly,Dite and Reindeer,which could be used as important germplasm resources for breeding and atrazine-tolerance research in alfalfa.