小叶章DNA甲基转移酶基因家族鉴定与分析

Identification and Analysis of DNA Methyltransferase Gene Family in Deyeuxia angustifolia Kom

DNA甲基化是一种高度保守的表观遗传修饰,涉及许多生物过程,在植物界,胞嘧啶DNA甲基转移酶基因在一些植物物种中已被鉴定。本研究在全基因组水平上对小叶章中的DNA甲基转移酶基因进行鉴定和分析,结果在小叶章中鉴定了29种DNA甲基转移酶,分为四个亚家族,分别是DaMET、DaCMT、DaDRM和DaDNMT2。基于这些基因的保守结构域分析,DNA甲基转移酶在进化过程中发生了基因丢失和复制事件。在DNA甲基转移酶中发现了多个顺式作用元件,包括光响应、植物激素响应、胁迫响应和植物生长发育相关的元件。此外,还研究了不同生境(典型草甸、沼泽、沼泽化草甸)下DNA甲基转移酶的转录丰度,大多数DaMET基因在不同生境下的表达水平均显著下调,而2个DaDRM基因的转录丰度均显著诱导。

DNA methylation is a highly conserved epigenetic modification concerning many biology processes.In plants,cytosine DNA methyltransferase genes have been identified in several plant species.In this study,DNA methyltransferase genes in Deyeuxia angustifolia Kom.was identified and analysed on a genome-wide level.29 DNA methyltransferases were identified in Deyeuxia angustifolia Kom..They were divided into four subfamilies,DaMET,DaCMT,DaDRM and DaDNMT2 respectively.Based on the analysis of the conserved structural domains of these genes,DNA methyltransferases gene loss and replication events occurred during the evolutionary process.In addition,multiple cis-acting elements were identified in DNA methyltransferases,including light response,phytohormone response,stress response and plant growth and development-related elements.Besides,the transcriptional abundance of DNA methyltransferases under different habitat(typical meadow,swamp,and marshy meadow)conditions was studied.Most DaMET expression levels were significantly down-regulated in different habitats,while transcript abundance of both DaDRM genes were significantly induced.