小麦旗叶面积相关性状的全基因组关联分析

Genome-wide Association Analysis of Flag Leaf Area Related Traits in Wheat

为了挖掘控制小麦旗叶面积相关性状的基因位点,本研究以215份适宜在冀中北地区种植的小麦品种(系)为研究材料,分别于2020—2022年在河北省唐山市种植,利用小麦16K高密度SNP芯片进行全基因组扫描,并结合小麦旗叶面积相关性状(旗叶宽、旗叶长、旗叶面积)进行全基因组关联分析。结果表明,有23个与小麦旗叶宽显著相关的SNP位点,其中位于1A、2B(6)、3A、4A、4B、5A、5B(5)、5D、6B染色体上的18个SNP位点(区段)在多个环境中被检测到;两个与旗叶长显著关联的SNP位点未在多个环境中被检测到;在关联出的21个与旗叶面积显著关联的SNP位点(区段)中,位于2B、5A染色体的两个位点(区段)在多个环境中被检测到。有13个SNP位点(区段)表现“一因多效”,分布于1A、2B(3)、3A、4A、5A(2)、5B(3)、5D、7A,这些位点在与旗叶面积显著关联的同时,也与旗叶宽或旗叶长呈显著关联。

In order to explore the gene locus controlling flag leaf area related traits of wheat,215 wheat varieties or lines suitable for northern Hebei Province were used as research materials,which were planted from 2020 to 2022,respectively.The whole-genome scanning using wheat 16K high density SNP chip was conducted.Genome-wide association analysis(GWAS)was conducted based on flag leaf area related traits(flag leaf width,length and area).The results showed that there were 23 SNPs significantly associated to flag leaf width,among which 18 SNPs on chromosomes 1A,2B(6),3A,4A,4B,5A,5B(5),5D and 6B were detected in multiple environments.Two SNPs significantly associated with flag leaf length were not detected in multiple environments.Among the 21 SNPs significantly associated with flag leaf area,two located on chromosomes 2B and 5A were detected in multiple environments.There were 13 SNPs showing a single cause and multiple effects on chromosomes 1A,2B(3),3A,4A,5A(2),5B(3),5D and 7A,and these SNPs were significantly associated with flag leaf area,as well as flag leaf width or length.