直接进样-气相色谱质谱法快速测定芝麻香白酒及酒醅中吡嗪类风味组分的研究

Rapid Determination of Pyrazines in Zhimaxiang Baijiu and Fermented Grains by Direct Injection GC-MS

本研究建立了直接进样-气相色谱质谱法(GC-MS)测定芝麻香白酒中8种吡嗪类化合物,该方法经CP-WAX色谱柱对样品进行分离后进行质谱分析,采用SIM模式,以保留时间和特征离子的丰度比定性,内标法定量。结果显示,8种吡嗪类风味组分的线性相关系数均大于0.999,该方法检出限为10μg/L,加标回收率为87%~110%,相对标准偏差为3%~10%。此方法采用内标法定量,灵敏度可靠,适用于芝麻香白酒及其酒醅中吡嗪风味组分的快速检测。

A direct injection gas chromatography-mass spectrometry(GC-MS)method was established for the determination of eight pyrazines in Zhimaxiang Baijiu.The CP-WAX column was used to separate the samples,and then mass spectrometry analysis was conducted.The SIM mode was used for qualitative determination by the retention time and the abundance ratio of characteristic ions,and the internal standard method was used for quantitative determination.The results showed that the linear correlation coefficients of the eight pyrazines were greater than 0.999;the detection limit of the method was 10μg/L,the spiked recovery rate was between 87%and 110%,and the relative standard deviation was between 3%and 10%.The method is sensitive and suitable for the rapid detection of pyrazines in Zhimaxiang Baijiu and its fermented grains.