生物炭联合丛枝菌根真菌(AMF)促进了大豆幼苗生长及光合能力

Effects of biochar combined with arbuscular mycorrhizal fungi on soybean seedling growth

大豆是世界上最重要的油料作物,为研究生物炭与丛枝菌根真菌联合施用对大豆幼苗生长的影响,设计室内盆栽试验,种植大豆幼苗,试验设计接种或不接种根内根孢囊霉(Rhiaophagus intraradices,RI)并施加0%、2%、5%(w/w)的生物炭,共6个处理。结果表明,接种RI并施加生物炭,可显著提高大豆幼苗的菌根侵染率、株高、茎粗和生物量,并显著改善了大豆幼苗的光合气体交换活性和叶绿素荧光特性,促进了大豆幼苗的生长发育,其中以接种RI并施加2%生物炭组合处理促进效应最大,菌根侵染率最高达到76.67%,大豆幼苗的株高、茎粗和总生物量与CK相比分别提高了86.10%、35.69%和80.00%。联合施加RI与2%生物炭的大豆幼苗的光合气体交换参数及叶绿素荧光参数显著优于CK组(P<0.05),说明RI与2%生物炭联合施用能有效增强大豆幼苗的光合能力。因此,RI与2%生物炭配施具有更好的菌根效应,能有效促进大豆幼苗的生长,这对大豆幼苗的生长及可持续农业发展具有重要意义。

In order to study the effects of combined application of biochar and arbuscular mycorrhizal fungi on the growth of soybean seedlings,a soybean pot experiment was designed.Rhiaophagus intraradices(RI)was either inoculated or not inoculated,and 0%,2%,5%(w/w)of biochar was applied in a total of 6 treatments.The results showed that RI inoculation and 2%biochar combined treatment had the greatest promoting effect,the highest mycorrhizal infection rate was 76.67%,the total biomass of soybean seedlings was increased by 80.00%compared with CK,the photosynthetic gas exchange parameters and chlorophyⅡfluorescence parameters were significantly better than CK group(P<0.05).It is indicated that RI combined with 2%biochar can effectively improve the photosynthetic capacity of soybean seedlings and promote the growth of soybean seedlings,which is of great significance for the sustainable development of soybean agriculture in China.