固定化米根霉全细胞催化α-蒎烯环氧化反应

Catalysis of immobilised whole-cells Rhizopus oryzae inα-pinene epoxidation reaction

为推动α-蒎烯的绿色环氧化,同时降低反应成本并提高催化剂的重用性,实验研究了使用固定化全细胞Rhizopus oryzae CGMCC 3.5040催化α-蒎烯环氧化反应的可行性,并采用单因素实验法对反应条件进行优化。结果表明:150 mg的固定化细胞添加量,有机相与30%H_(2)O2溶液的体积比为3∶1,1.5 mmol的辛酸,反应8 h后,α-蒎烯的转化率可达到(99.7±0.1)%,2,3-环氧蒎烷的收率为(95.1±1.1)%。此外,对固定化全细胞催化剂进行重复利用,发现使用甲苯作为清洗剂可以有效保持催化剂的稳定性,催化剂在循环使用7次后仍能保留75.0%以上的转化率。这项研究为环境友好的催化方法提供了一种新思路,也为工业上的化学合成提供了一种新的选择。

In order to promote the green epoxidation ofα-pinene,while reducing the reaction costs and improving the reusability of the catalysts,the feasibility of using immobilized whole-cells Rhizopus oryzae CGMCC 3.5040 to catalyze the epoxidation ofα-pinene was studied in this experiment.The reaction conditions were optimized by using a single-factor experimental method.The results show that the conversion ofα-pinene and the yield of 2,3-epoxypinene are(99.7±0.1)%and(95.1±1.1)%,respectively,after reacting 8 h with 150 mg of immobilized cell addition,the volume ratio 31 of organic phase to 30%H_(2) O 2 solution and 1.5 mmol of octanoic acid.In addition,the immobilized whole-cell catalysts were reused.It is found that using toluene as a cleaning agent can effectively maintain the stability of the catalysts and the catalysts retain more than 75.0%conversion rate after seven cycles.This study provides a new idea for an environmentally friendly catalytic approach and a new option for chemical synthesis in industry.