桉树组培苗生产污染菌的调查与分析

Investigation and Analysis of Contaminant Microorganisms in Eucalypt Tissue Culture Seedling Production

采用形态学和分子生物学方法对桉树组培苗污染菌的种类进行了调查,为减少桉树组培苗生产污染提供依据。结果表明:污染菌主要包括25个属50个种,其中污染细菌6个属7个种,污染真菌19个属43个种,细菌、真菌分别占14.0%、86.0%。继代苗的污染菌主要是丽水黄色杆菌(Luteibacter yeojuensis)和青霉属(Penicillium spp.)、曲霉属(Aspergillus spp.)各5个种;外植体的污染菌为11属21个种的真菌和1个属1个种的细菌。

Species of contaminated bacteria found in eucalypt tissue cultures were investigated by morphological and molecular biology methods in order to potentially reduce the incidence of deleterious contamination of such cultures.In total 50 species representing 25 genera of microorganism were identified from tissue culture bottles,including 7 species from 6 genera of bacteria along with 43 species from 19 genera of fungi,equating to 14.0%of contaminants being bacteria and 86.0%being fungi.Eucalypt subculture seedlings were mainly contaminated by Luteibacter yeojuensis along with Penicillium spp.and Aspergillus spp.each with 5 species.Later after transplanting,it was found that eucalypt explants were contaminated by 21 species representing 11 genera of fungi and 1 species of bacteria.