pre-S基因变异与乙型肝炎病毒感染患者肝功能和肝纤维化的相关性

Correlation between pre-S gene mutations with liver function and liver fibrosisin patients with hepatitis B virus infection

目的 研究了pre-S基因变异与乙型肝炎病毒(HBV)感染患者肝功能和肝纤维化的相关性。方法 选取2019年12月至2022年6月该院收治的HBV感染者178例为研究对象,其中无症状HBV携带患者65例作为对照组,慢性乙型肝炎(CHB)患者70例作为CHB组,乙型肝炎肝硬化患者43例作为肝硬化组。比较对照组、CHB组、肝硬化组患者的pre-S基因变异情况、肝功能和肝纤维化指标水平。根据是否发生基因缺失变异,将HBV感染患者分为变异组和非变异组,比较变异组和非变异组的肝功能和肝纤维化指标水平。采用Spearman相关分析pre-S基因变异类型与患者肝功能和肝纤维化指标的相关性。结果 对照组和CHB组pre-S基因缺失变异率明显低于肝硬化组,CHB组pre-S基因缺失变异率明显低于肝硬化组,差异均有统计学意义(P<0.05)。对照组和CHB组ALT、AST、层粘连蛋白、透明质酸以及Ⅲ型前胶原肽水平明显低于肝硬化组,清蛋白水平明显高于肝硬化组,差异均有统计学意义(P<0.05)。非变异组ALT、AST、层粘连蛋白、透明质酸以及Ⅲ型前胶原肽水平明显低于变异组,清蛋白水平明显高于变异组,差异均有统计学意义(P<0.05)。pre-S基因缺失变异与ALT、AST、层粘连蛋白、透明质酸、Ⅲ型前胶原肽水平均呈正相关(P<0.05),与清蛋白水平呈负相关(P<0.05)。结论 pre-S基因缺失变异与HBV感染患者肝功能和肝纤维化指标明显相关。

Objective To study the correlation between pre-S gene mutations with the liver function and liver fibrosis in the patients with hepatitis B virus(HBV)infection.Methods A total of 178 patients with HBV infection admitted to this hospital from December 2019 to June 2022 were selected as the study subjects,among them 65 asymptomatic carriers with HBV were included in the control group,70 patients with chronic hepatitis B(CHB)were included in the CHB group,and 43 patients with hepatitis B cirrhosis were included in the cirrhosis group.The pre-S gene mutations,liver function and liver fibrosis indexes levels were compared among the control group,CHB group and liver cirrhosis group.According to the occurrence of gene deletion mutations,the patients with HBV infection were divided into the mutation group and non-mutation group,and the liver function and liver fibrosis indexes levels were compared between the mutation group and non-mutation group.The Spearman correlation was used to analyze the correlation between pre-S gene variant type with the liver function and liver fibrosis indicators of the patients.Results The pre-S gene deletion mutation rate in the control group and CHB group were significantly lower than that in the liver cirrhosis group,the pre-S gene deletion mutation rate in the CHB group was significantly lower than that in the liver cirrhosis group,and the differences were statistically significant(P<0.05).The levels of ALT,AST,laminin,hyaluronic acid and typeⅢprocollagen peptide in the control group and CHB group were significantly lower than those in the liver cirrhosis group,while the albumin level was significantly higher than that in the liver cirrhosis group,and the differences were statistically significant(P<0.05).The levels of ALT,AST,laminin,hyaluronic acid and typeⅢprocollagen peptide in the non-mutation group were significantly lower than those in the mutation group,while the albumin level was significantly higher than that in the mutation group(P<0.05).The pre-S gene deletion mutation was positively correlated with the levels of ALT,AST,laminin,hyaluronic acid and typeⅢprocollagen peptide(P<0.05),and negatively correlated with the albumin level(P<0.05).Conclusion The pre-S gene deletion mutation has significantly correlated with the liver function and liver cirrhosis indicators in the patients with HBV infection.