摘要
The dehalogenation of organohalides has been a research hotspot in bioremediation field;however,the influence of tourmaline,a natural ore that can generate spontaneous electric field,on organohalide-respiring bacteria(OHRB)and their dechlorination process is not well known.In this study,the effect and mechanism of tourmaline on the reductive dechlorination of 2,3-dichlorophenol(2,3-DCP)by Desulfitobacterium hafniense DCB-2Twere explored.The characterization results confirmed that tourmaline had good stability and the optimal dosage of tourmaline was 2.5 g/L,which shortened the total time required for dechlorination reaction to 72 hr.Besides,tourmaline amendment also increased the proportion of 2-chlorophenol(2-CP)from 18%to 30%of end products,while that of 3-CP decreased correspondingly.The theoretical calculations showed that the bond charge of the orthosubstituted chlorine declined from-0.179 to-0.067,and that of meta-substituted chlorine increased from-0.111 to-0.129,which indicated that the spontaneous electric field of tourmaline affected the charge distribution of 2,3-DCP and was more conducive to the generation of 2-CP.Overall,tourmaline with the spontaneous electric field affected the reductive dechlorination pathway of Desulfitobacterium,and the tourmaline-OHRB combining system might serve as a novel strategy for the bioremediation of environments polluted with chlorinated phenols.
基金
supported by the Natural Science Foundation of Hunan Province of China (No.2020JJ4194)
the Shenzhen Science and Technology Program (No.JCYJ20220530160412027)
the Changsha Science and Technology Program (No.kq2004022)
the Science and Technology Innovation Program of Hunan Province (No.2022RC1026)
the Project of the National Key Research and Development Program of China (No.2021YFC1910400)
the Technical Innovation Leading Plan Project for Hunan High-tech Industry (Nos.2020SK2042 and2022GK4062)
the Key R&D Project of Hunan Province of China (No.2022SK2067)。