常山胡柚果实不同部位中主要功能性成分分析

Analysis of main functional components in different parts of Citrus×aurantium L.Changshanhuyou fruits

本研究针对常山胡柚全果油胞层、白皮层、囊衣、汁胞和籽5个不同部位,连续提取其中的多酚类物质和果胶多糖,采用超高效液相色谱-串联质谱法测定不同部位中的30种柑橘多酚类物质,比较果胶多糖的组成及分子量,全面分析常山胡柚果实不同部位中主要功能性成分的组成。结果表明:常山胡柚各部位黄酮类化合物主要以黄烷酮类形式存在,白皮层中新橙皮苷、新北美圣草苷质量分数较高,分别可达13700.72μg/g(按干质量计,下同)和6270.24μg/g;不同部位酚酸类组成中肉桂酸类含量高于苯甲酸类,且以油胞层中肉桂酸类含量最高;淀粉酶辅助乙二胺四乙酸溶液提取到的白皮层果胶多糖得率最高(27.46%),氢氧化钠(NaOH)溶液提取到的油胞层果胶多糖得率最高(11.30%),且2种提取方法得到的油胞层果胶多糖重均分子量均最大,分别为6.888×10^(5)g/mol和2.343×10^(5)g/mol。常山胡柚油胞层、白皮层和囊衣可作为黄酮类化合物和果胶的加工来源,其丰富的新北美圣草苷可作为胡柚加工副产物新的开发方向。

In this study,the whole fruit of Citrus×aurantium L.Changshanhuyou was divided into five parts,namely,the flavedo,albedo,segment membrane,juice sacs and seeds,from which polyphenols and pectin polysaccharides were extracted sequentially.Ultra-high performance liquid chromatography-tandem mass spectrometry was used to determine 30 kinds of citrus polyphenols,and the compositions and molecular weights of pectin polysaccharides were compared to comprehensively analyze the compositions of the main functional components in different parts of Citrus×aurantium L.Changshanhuyou fruits.The results showed that flavonoids in various parts mainly existed in flavanones,and the contents of neohesperidin and neoeriocitrin in the albedo were the highest,reaching 13700.72μg/g and 6270.24μg/g(by dry mass and the same as below),respectively.The contents of cinnamic acids in various parts were higher than that of benzoic acids,and the contents of cinnamic acids in the flavedo were the highest.The yield of pectin polysaccharides extracted from albedo by amylase-assisted ethylenediamine tetraacetic acid solution was the highest(27.46%).The yield of pectin polysaccharides from the flavedo by the extraction of NaOH solution was the highest(11.30%),and the molecular weights(MW)of pectin polysaccharides extracted by the two extraction methods in the flavedo were up to 6.888×105 g/mol and 2.343×105 g/mol,respectively.The flavedo,albedo and segment membrane of Citrus×aurantium L.Changshanhuyou can be used as the main processing sources of flavonoids and pectin,and their rich contents of neoeriocitrins provide new development direction for processing byproducts.