黄绿卷毛菇中原伊鲁烷型倍半萜芳基酯提取工艺优化及活性研究

Optimization of extraction process and activity of protoilludane sesqui terpene aryl esters from Floccularia luteovirens

本研究探讨了不同提取工艺对黄绿卷毛菇中原伊鲁烷型倍半萜芳基酯提取效果的影响。采用超声波辅助提取法,在单因素实验基础上,应用Box-Behnken响应面法优化提取工艺条件,同时考察原伊鲁烷型倍半萜芳基酯的体外抗氧化活性,并采用抑菌圈法评估其对3种常见致病菌的抑菌活性。结果表明:乙酸乙酯为黄绿卷毛菇中原伊鲁烷型倍半萜芳基酯的最佳提取溶剂,在料液比为1∶33(质量体积比)、超声时间为33 min、超声功率为166 W的条件下,原伊鲁烷型倍半萜芳基酯提取量达到10.107μmol/g。此外,原伊鲁烷型倍半萜芳基酯具有较高的清除2,2-联苯基-1-苦基肼基自由基与羟自由基的能力,并对大肠埃希菌、金黄色葡萄球菌、蜡样芽孢杆菌具有不同程度的抑菌效果。本研究为进一步开发黄绿卷毛菇相关产品提供了数据支持与理论依据。

In this study,the effects of different methods on the extraction of protoilludane sesquiterpene aryl esters from Floccularia luteovirens were studied.Using the ultrasonic-assisted extraction method,the extraction process conditions were optimized by Box-Behnken response surface methodology based on a single-factor experiment.The in vitro antioxidant activity of protoilludane sesquiterpene aryl esters was determined,and the antibacterial activities against three common pathogenic bacteria were evaluated via the inhibition zone method.The results showed that ethyl acetate was the most suitable solvent for the extraction of protoilludane sesquiterpene aryl esters.The optimal extraction conditions for accessing protoilludane sesquiterpene aryl esters from F.luteovirens were as follows:solid-liquid ratio of 1∶33(mass to volume),ultrasonic time of 33 min,and ultrasonic power of 166 W.Under these conditions,the extraction content of protoilludane sesquiterpene aryl esters was 10.107μmol/g.In addition,the protoilludane sesquiterpene aryl esters had strong scavenging effects against 2,2-diphenyl-1-picrylhydrazyl free radicals and hydroxyl free radicals,and had different antibacterial effects on Escherichia coli,Staphylococcus aureus,and Bacillus cereus.This study provides data support and a theoretical basis for the further development of F.luteovirens related products.