不同保存条件下血浆补体含量观察

Complement content in plasma under different storage conditions

目的探讨成分血在临床输注前补体含量变化。方法按照制备工艺、保存温度和时间段不同,将20人份相同来源的血浆在制备工艺不同阶段留样的180份标本集中在同批次进行实验检测,观察不同保存温度和工艺阶段分组条件下补体C3、C4含量变化。结果用同一样本检测C3、C4含量,将同源样本检测结果按照不同保存温度和制备工艺阶段整理分为九组进行比较。其中,C3:0.9941;C4:0.9571;P>0.05,单纯-40℃、-60℃不同温度保存差异不显著;C3:0.1333,0.22406,P>0.05;C4:0.0273,0.0251,P<0.05;4℃保存7d或可显著降低补体含量有待进一步验证;C3:0.0470,0.0383;C4:0.0426,0.0121;P<0.05,病毒灭活制备工艺能够显著降低补体含量,冰冻血浆加工过程中反复冻融能够改变补体含量。补体C3、C4含量测定实验相关性分析表明,P<0.05,二者在温度、制备工艺和保存时间等不同条件下的变化呈显著正相关。结论冰冻血浆保存温度、保存时间以及制备过程中反复冻融、离心浓缩、光照灭活等因素能够影响补体含量。

Objective To investigate the changes in complement content in component blood before blood transfusion.Methods 180 samples from 20 plasma donors were collected at different stages of the preparation process,stored at different temperatures and time periods,and tested in the same batch to observe the changes in complement C3 and C4 levels under different storage temperatures and process stages.Results The same sample was used to test C3 and C4 levels,and the test results were sorted into nine groups for comparison according to different storage temperatures and preparation process stages.C3:0.9941;C4:0.9571,with no significant difference in storage at-40℃and-60℃(P>0.05);C3:0.1333,0.22406(P>0.05);C4:0.0273,0.0251(P<0.05),storing at 4℃for 7 days may significantly reduce complement levels,which needs further verification;C3:0.0470,0.0383;C4:0.0426,0.0121(P<0.05),virus inactivation preparation process can significantly reduce complement levels,repeated freeze-thawing during frozen plasma processing can change complement levels.The correlation analysis of C3 and C4 content determination experiment shows that they are significantly positively correlated under different storage temperature,preparation process and storage time(P<0.05).Conclusion Storage temperature,storage time,preparation process of frozen plasma such as repeated freeze-thawing,centrifugal concentration and light inactivation have an impact on complement content.