盐度和温度对创伤弧菌生长、运动及生物膜形成的影响

Effects of Salinity and Temperature on the Growth,Motility and Biofilm Formation of Vibrio Vulnificus

研究盐度和温度对创伤弧菌(Vibrio Vulnificus)的生长、运动及生物膜形成是否具有调控作用.控制盐度(0.5%、1%、2%、3%和4%NaCl)和温度(37℃和26℃)条件,通过测定创伤弧菌在肉汤培养基(Luria-Bertani medium,LB)中的生长曲线,来分析盐度和温度对其生长的影响;将创伤弧菌接种于软琼脂平板上,通过比较不同盐度和温度下的菌苔直径差异,来分析盐度和温度对创伤弧菌运动能力的影响;通过结晶紫染色实验检测创伤弧菌在不同盐度和温度下的生物膜形成能力差异.创伤弧菌能够在0.5%~4%的盐浓度下生长,1%盐浓度是其最适宜生长盐度;1%盐浓度时创伤弧菌运动能力最强,而4%盐浓度时其生物膜形成能力最强;与26℃相比,37℃下创伤弧菌的生长、运动和生物膜形成能力均显著增强.盐度和温度能够调控创伤弧菌的生长、运动及生物膜形成.

This study investigates whether salinity and temperature regulate the growth,motility,and biofilm formation of Vibrio vulnificus.The effects of salinity(0.5%,1%,2%,3%,and 4%NaCl)and temperature(37℃and 26℃)on the growth of V.vulnificus in Luria-Bertani(LB)medium were analyzed by measuring growth curves.The impact of salinity and temperature on the motility of V.vulnificus was examined by comparing the diameters of colonies on soft agar plates under different salinity and temperature conditions.The ability to form biofilms at various salinities and temperatures was assessed using crystal violet staining.V.vulnificus can grow in salinity concentrations ranging from 0.5%to 4%NaCl,with 1%NaCl being the optimal salinity for growth.At 1%NaCl salinity,the motility of V.vulnificus is the strongest,while at 4%NaCl salinity,its biofilm formation ability is the strongest.Compared to 26℃,the growth,motility,and biofilm formation capabilities of V.vulnificus are significantly enhanced at 37℃.Salinity and temperature can regulate the growth,motility,and biofilm formation of V.vulnificus.