高压氧治疗对阿尔兹海默症大鼠认知功能的影响

Influence of hyperbaric oxygen therapy on cognitive function in rats with Alzheimer's disease

目的应用脑立体定位技术在双侧海马注射β-淀粉样蛋白片段25-35(Aβ25-35)的方法建立AD大鼠实验模型,探究高压氧治疗对AD大鼠学习记忆能力、空间探索能力及认知功能的影响。方法选用30只成年雄性SD大鼠,随机分成三组,每组各10只,分别是对照组(control组)、AD模型组、高压氧治疗组(HBO+AD组)。应用脑立体定位技术在双侧海马注射β-淀粉样蛋白片段25-35(Aβ25-35)建立AD大鼠模型。将10只AD模型鼠放置于动物高低压氧实验舱中进行5个周期的高压氧治疗,高压氧治疗后随即进行Morris水迷宫实验,记录在定位航行实验期间和空间探索实验期间大鼠经过原平台的次数、经过目标象限的次数,首次到达原平台的时间以及在目标象限停留的时间,观察高压氧治疗对各组大鼠学习记忆能力、空间探索能力及认知功能的影响。结果与AD模型组相比较,在为期5天的定位航行实验期间,高压氧治疗组大鼠逃避潜伏期时间显著减少(P<0.01),且运动轨迹逐渐向内环移动。在空间探索实验期间,与AD模型组相比较,高压氧治疗组大鼠经过平台的次数(P<0.05)、经过目标象限的次数(P<0.01)以及在目标象限停留的时间(P<0.01)均显著增加,首次到达平台的时间显著减少(P<0.05),差异具有统计学意义。结论通过立体定位技术在双侧海马注射β-淀粉样蛋白片段25-35(Aβ25-35)建立AD大鼠实验模型,经过高压氧治疗后,有效改善AD模型组大鼠学习记忆能力、空间探索能力及认知功能。

Objective An experimental model of AD rats was established by injecting Aβ25-35 in bilateral hippocampus through applying brain stereotaxic localization technique to investigate the effects of hyperbaric oxygen therapy on learning memory ability,spatial exploration ability and cognitive function of AD rats.Methods Thirty male adult SD rats were selected and randomly divided into 3 groups,with 10 rats in each group,which were control group(control),AD model group,and hyperbaric oxygen therapy group(HBO+AD).A rat model of AD was established by injectingβ-amyloid fragment 25-35(Aβ25-35)in bilateral hippocampus using brain stereotaxic localization technique.Ten AD model rats were placed in the animal hyperbaric oxygen chamber for 5 cycles of hyperbaric oxygen treatment,and the Morris water maze experiment was conducted immediately after the hyperbaric oxygen treatment.The number of times the rats passed the original platform,the number of times they passed the target quadrant,the time they reached the original platform for the first time,and the time they stayed in the target quadrant were recorded during the localization navigation experiment and the spatial exploration experiment.The effects of hyperbaric oxygen therapy on the learning memory ability,spatial exploration ability and cognitive function of rats in each group were observed.Results Compare with the AD model group,rats in the hyperbaric oxygen treatment group has a significantly reduced escape latency time(P<0.01)and a gradual movement trajectory toward the inner ring during the five-day localization navigation experiment.During the space exploration experiment,the number of times the rats in the hyperbaric oxygen treatment group passe through the platform(P<0.05),the number of times they passe through the target quadrant(P<0.01),and the time they stay in the target quadrant(P<0.01)are significantly increase,and the time to reach the platform for the first time is significantly decrease(P<0.05),and the differences were statistically significant.Conclusions An experimental model of AD rats are successfully established by injecting Aβ25-35 in bilateral hippocampus by stereotactic technique,and after hyperbaric oxygen treatment,the learning memory ability,spatial exploratory ability,and cognitive function of rats in the AD model group are effectively improved.