甘蓝广谱抗根肿病材料的鉴定和创制

Identification and Creation of Broad-spectrum Clubroot Resistance Materials

甘蓝根肿病是由芸薹根肿菌(Plasmodiophora brassicae Woronin)侵染引发的土传病害,严重影响甘蓝的生产。由于根肿菌生理小种比较复杂,因此广谱抗根肿病甘蓝材料较少。本试验以湖北长阳、重庆武隆、四川广元的根肿菌为病原菌菌源,采用孢子注射法进行接种,对抗根肿病Ogura CMS甘蓝材料GY306进行抗性鉴定。结果表明,GY306对3个地区根肿菌生理小种的抗性表现分别为免疫(DI=0)、抗病(DI=6.25)和免疫(DI=0)。同时,利用Ogura CMS育性恢复材料对GY306进行育性恢复,通过根肿病抗性鉴定筛选获得了3份抗根肿病甘蓝材料。

Clubroot is a soil-borne disease caused by Plasmodiophora brassicae,which seriously threatens the production of cabbage.Due to the complex race differentiation of P.brassicae,there are fewer broad-spectrum resistance materials to clubroot.In this study,the resistance of cytoplasmic sterile clubrootresistant material‘GY306’was identified by spore injection method.The results indicated that‘GY306’was resistant to all P.brassicae from 3 different regions,and the disease grade indexes were immune(Changyang,DI=0),disease resistance(Wulong,DI=6.25)and immunity(Guangyuan,DI=0)respectively.At the same time,the fertility of‘GY306’was restored by crossing with Ogura CMS fertility recovery material.Finally,three clubroot resistance materials were obtained by identifying and screening the resistance of restored plants.