辣椒轻斑驳病毒RT-LAMP快速检测方法的建立

Establishment of a Rapid RT-LAMP Detection Method for Pepper Mild Mottle Virus

辣椒轻斑驳病毒(pepper mild mottle virus,PMMo V)是发生在辣椒上的一种重要病毒病。通过逆转录环介导等温扩增技术(RT-LAMP),以PMMo V外壳蛋白CP序列为靶标基因,设计F3/B3、FIP/BIP 4条引物,构建了基于RT-LAMP技术的PMMo V快速检测方法。结果表明,该方法在60 min内便可完成对PMMo V的特异性检测,灵敏度比传统RT-PCR技术高10倍,检测过程中与其他相关病毒无交叉反应。本试验建立的PMMo V RT-LAMP检测方法操作简便、特异性强、灵敏度高,可用于田间生产中对PMMo V的快速检测。

Pepper mild mottle virus(PMMoV)is a significant viral disease that occurs in peppers.In this study,we utilized reverse transcription loop-mediated isothermal amplification technology(RTLAMP)to develop a rapid PMMoV detection method.Specifically,we targeted the PMMoV coat protein CP sequence and designed four primers(F3/B3 and FIP/BIP).The results demonstrate this method enables specific detection of PMMoV within 60 minutes,offering a sensitivity 10 times higher than traditional RT-PCR technology.Furthermore,it does not exhibit any cross-reaction with other related viruses during the detection process.The established PMMoV RT-LAMP detection method is characterized by its simplicity,strong specificity,and high sensitivity,making it suitable for rapid detection of PMMoV in field production.