基于SSR标记的芡实遗传多样性分析及指纹图谱构建

Genetic Diversity Analysis and Fingerprinting Construction of Euryale ferox Based on SSR Markers

为进一步明确我国芡实种质资源的遗传背景,采用SSR标记扩增和籽粒品质性状测定,对10份芡实材料进行了遗传多样性分析。基于10对SSR引物对10份芡实材料的扩增及检测结果,共获得29个位点,其中28个具有多态性,多态百分率为96.6%,品系间Nei&Li相似系数平均值为0.5803,以相异系数0.57为阈值可将所有材料分为4组;基于籽粒品质性状测定结果,品系间欧式遗传距离平均值为4.0575,以欧氏遗传距离3.50为阈值可将所有材料分为3组。试验结果明确了现有芡实材料之间的遗传距离,构建了芡实种质资源DNA指纹图谱。

In order to elucidate the genetic background of Euryale ferox resources in china,10 germplasm samples of E.ferox were used as materials to analyze genetic diversity and relationships by SSR marker and seed quality.Based on the amplification and detection results of 10 SSR primer pairs across the 10 materials,a total of 29 bands and 28 polymorphic bands were amplified,with a polymorphism percentage of 96.6%.In cluster analysis,the average Nei&Li similarity coefficient was 0.5803,and 10 materials were divided into 4 groups at the genetic coefficient of 0.57.Utilizing seed quality trait analysis,the average Euclidean genetic distance among 10 germplasm was 4.0575,and 10 materials were divided into 3 groups at the Euclidean genetic distance of 3.50.This study clarified the genetic distance between existing E.ferox resources and the DNA fingerprint of 10 E.ferox materials were constructed by SSR markers.