Sysmex XN系列血细胞分析仪与显微镜镜检对白细胞分类计数百分比检测中的对比分析

Comparison and Analysis of Sysmex XN Series Hematology Analyzer and Microscopic Examination in the Detection of the Percentage of Leukocyte Classification Count

目的:评价Sysmex XN系列血细胞分析仪(仪器法)与显微镜镜检(镜检法)对白细胞分类计数的准确性,为临床标本检查的规范化提供依据。方法:选择90份用仪器法检测血常规结果出现白细胞分类计数有报警提示的标本,同时进行显微镜镜检(镜检法)复检,比较仪器法和金标准镜检法的结果。结果:送检的90例血清样本,有62例为真阳性,符合率为68.89%;28例呈现假阳性,假阳性率占31.11%。仪器法检测存在单核细胞假性升高,中性粒细胞假性减低现象。结论:Sysmex XN系列血细胞分析仪白细胞出现报警提示可以有效筛选阳性标本,但同时对阳性标本需要进行人工镜检复核,以避免假阳性结果。

Objective:To evaluate the accuracy of Sysmex XN series blood cell analyzer(instrument method)and microscopic examination(microscopic examination method)in the classification and counting of leukocytes,so as to provide a basis for the standardization of clinical specimen examination.Methods:Select 90 specimens with alarm prompts for white blood cell classification count detected by instrumental method in blood routine testing,and perform microscopic examination(microscopic examination)at the same time to compare the results of instrumental method and gold standard microscopic examination.Results:Of the 90 samples,62 were true positive,with a coincidence rate of 68.89%;28 cases were false positive,the false positive rate was 31.11%.Instrument method showed that there was a false increase in monocytes and a false decrease in neutrophils.Conclusion:The alarm prompt of white blood cells in Sysmex XN series blood cell analyzer can effectively screen positive samples,but at the same time,the positive samples need to be rechecked by manual microscopy to avoid false positive results.