杜仲腰痛丸对大鼠脊髓损伤后炎症反应抑制的影响

Efficacy of Duzhong Yaotong Wan on inhibition of inflammatory response after spinal cord injury in rats

目的:观察杜仲腰痛丸对大鼠脊髓损伤后炎症反应及神经元细胞形态变化的影响,探讨杜仲腰痛丸对脊髓损伤的保护作用及可能机制。方法:将50只成年SD大鼠随机分为A组、B组、C组,其中C组(药物干预)根据给药剂量不同依次分为C1组、C2组、C3组,共5组,每组各10只。A组行椎板切除术(不损伤脊髓),B组和C组均建立脊髓钳夹压迫损伤模型。造模成功后,A组和B组给予0.9%氯化钠溶液灌胃,C组给予不同剂量杜仲腰痛丸药液灌胃。分别于术后第1天、第3天、第5天、第7天进行BBB(Basso,Beattie and Bresnahan)肢体运动功能评分;于术后第7天取材后采用尼氏染色以观察脊髓组织病理变化,酶联免疫吸附试验(Enzyme-linked Immunosorbent Method,ELISA)法以检测肿瘤坏死因子-β和白细胞介素-1表达。结果:BBB肢体运动功能评分比较,术后第3天起C组显著优于同期B组及A组,差异具有统计学意义(P<0.05)。尼氏染色观察比较,C组根据给药剂量由低到高瘀血面积依次减小,神经细胞水肿程度依次减轻。ELISA检测分析,与A组及B组相比较,C组血清中肿瘤坏死因子-β和白细胞介素-1含量明显减低,差异具有统计学意义(P<0.05)。结论:杜仲腰痛丸能够有效抑制脊髓损伤后炎症反应,对神经元细胞具有保护作用,促进损伤神经细胞的修复。

Objective:To observe the effects of Duzhong Yaotong Wan(杜仲腰痛丸)on inflammatory response and neuronal cell morphological changes after spinal cord injury in rats,and to explore the protective effect and possible mechanism of Duzhong Yaotong Wan on spinal cord injury.Methods:Fifty adult SD rats were randomly divided into the group A,the group B and the group C.The intervention group successively divided them into the group C1,the group C2 and the C3 group according to different dose,with 10 rats in each group.Laminectomy was performed in the group A(without spinal cord injury),and spinal cord clamp injury model was established in the group B and the group C.After modeling,the group A and the group B were given normal saline intragastric administration,while the group C was given different doses of Duzhong Yaotong Wan liquid of intragastric administration.BBB limb motor function were performed to grade on the 1st day,3 rd day,5th day and 7th day after surgery respectively.Nissl staining was used to observe the pathological changes of the spinal cord on the 7th postoperative day,and TNF-βand IL-1 expression were detected by ELISA.Results:By using BBB limb motor function to compare the scores,from the 3 rd day after surgery the group C were significantly better than the group B and the group A with the corresponding period.The difference was statistically significant(P<0.05).By using Nissl stain to observe and compare,in the group C,the area of blood stasis decreased from low to high according to the dose,and the degree of nerve cell edema decreased successively.ELISA analysis showed that compared with the group A and the group B,serum TNF-βand IL-1 contents in the group C were significantly decreased,and the difference was statistically significant(P<0.05).Conclusion:Duzhong Yaotong Wan can effectively inhibit inflammatory response,have protective function to neuronal cells,and promote the repair of injured nerve cells.