miR-200b、miR-200c靶向肝细胞生长因子抑制结直肠癌细胞

Mir-200b,mir-200c Targeted Hepatocyte Growth Factor Inhibitting Colorectal Cancer Cells

目的研究miR-200b、miR-200c对肝细胞生长因子进行靶向调节抑制结直肠癌细胞增殖的机制。方法采用生物信息学软件预测和验证miR-200b、miR-200c靶基因,分析miR-200b、miR-200c过表达抑制HGF表达,并分析miR-200b、miR-200c在体外影响结直肠癌细胞增殖活性的情况。结果培养1d、2d、3d,阴性对照模拟物的结直肠癌细胞增殖活性均高于miR-200b模拟物、miR-200c模拟物、miR-200b/c模拟物(P<0.05),阴性对照抑制剂的结直肠癌细胞增殖活性均低于miR-200b抑制剂、miR-200c抑制剂、miR-200b/c抑制剂(P<0.05),miR-200b模拟物、miR-200c模拟物的结直肠癌细胞增殖活性均高于miR-200b/c模拟物(P<0.05),miR-200b抑制剂、miR-200c抑制剂的结直肠癌细胞增殖活性均低于miR-200b/c抑制剂(P<0.05)。结论miR-200b、miR-200c对肝细胞生长因子进行靶向调节抑制结直肠癌细胞增殖,可以作为潜在治疗靶点。

Objective To investigate mechanism of miR-200b and miR-200c targeted regulation of liver cell growth factor inhibiting proliferation of colorectal cancer cells.Methods The paper predicted and validated target genes of miR-200b and miR-200c with bioinformatics software,analyzed overexpression of miR-200b and miR-200c to inhibit HGF expression,and effect of miR-200b and miR-200c on proliferation activity of colorectal cancer cells in vitro.Results cultured on 1d,2d,and 3d,proliferation activity of colorectal cancer cells in negative control analog was higher than miR-200b analog,miR-200c analog,and miR-200b/c analog(P<0.05).Proliferation activity of colorectal cancer cells in negative control inhibitor was lower than miR-200b inhibitor,miR-200c inhibitor,and miR-200b/c inhibitor(P<0.05).Proliferation activity of colon cancer cells in miR-200c mimetics was higher than miR-200b/c mimetics(P<0.05),while proliferation activity of colon cancer cells in miR-200b inhibitors and miR-200c inhibitors was lower than miR-200b/c inhibitors(P<0.05).Conclusion MiR-200b and miR-200c targeted regulation liver cell growth factors can inhibit proliferation of colorectal cancer cells,which can serve as potential curative targets.